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oai:www.ideals.illinois.edu:2142/101694 2023-07-11 col_2142_5131 com_2142_5130

Smith-Bolton, Rachel K Stubbs, Lisa J. Mizzen, Craig Raetzman, Lori T. Schuster, Keaton Jay 2018-09-27T16:30:28Z 2018-09-27T16:30:28Z 2020-09-28T09:15:24Z 2018-07-10 2018-08 Regenerating tissue must replace lost structures with cells of the proper identity and pattern in order to restore function. This thesis will describe two major insights into how patterning and cell fate is maintained and restored during the late phases of regeneration in the wing imaginal disc of Drosophila. First, the identification of taranis as a regeneration-specific patterning gene and its subsequent characterization as a factor that is required to protect the regenerating cells in the wing imaginal disc from inappropriate posterior to anterior cell fate changes that are induced by the powerful JNK signaling cascade at the wound misregulating the expression of engrailed. The other chapter will detail the identification of the pioneer transcription factor Zelda as being upstream of taranis expression during regeneration. Zelda is found to be expressed at the same place and time as Taranis, and reduction of Zelda levels results in profound anterior and posterior patterning defects. Speculation is provided suggesting that Zelda may also be essential for the large developmental transition from a program devoted to regenerative growth to the repatterning phase that allows for the restoration of cell fate and patterning genes that was lost earlier in regeneration. This work describes identification of a novel gene regulatory network essential for patterning and cell fate during regeneration. Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01 The student, Keaton Schuster, accepted the attached license on 2018-07-10 at 12:45. The student, Keaton Schuster, submitted this Dissertation for approval on 2018-07-10 at 15:14. This Dissertation was approved for publication on 2018-07-10 at 16:18. DSpace SAF Submission Ingestion Package generated from Vireo submission #12794 on 2018-09-27 at 11:18:42 Made available in DSpace on 2018-09-27T16:30:28Z (GMT). No. of bitstreams: 3 SCHUSTER-DISSERTATION-2018.pdf: 5459441 bytes, checksum: 6a6e6a0e4d779b5dafaa9eb0cd866f1d (MD5) LICENSE.txt: 4212 bytes, checksum: 8161a456cee2a0dba913c21425c61ab0 (MD5) PROQUEST_LICENSE.txt: 4558 bytes, checksum: 5d7946b65bf88c7d23dff78344451674 (MD5) Previous issue date: 2018-07-10 Embargo set by: Seth Robbins for item 107792 Lift date: 2020-09-27T16:30:34Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 107792 Lift date: 2020-09-27T16:31:43Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 107792 Lift date: 2020-09-27T16:34:29Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system U of I Only Restriction Lifted for Item 107792 on 2020-09-28T09:15:24Z. application/pdf http://hdl.handle.net/2142/101694 en Copyright 2018 Keaton Schuster Regeneration, Cell Fate, Patterning, Drosophila The guardians of cell fate: protective mechanisms that ensure proper cell fate and patterning during imaginal disc regeneration in drosophila text text Cell & Developmental Biology Cell and Developmental Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.