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oai:www.ideals.illinois.edu:2142/101749 2023-07-11 col_2142_5131 col_2142_14781 com_2142_5130 com_2142_687 com_2142_397

Pan, Yuan-Xiang Cai, Mingzhu 2018-09-27T16:45:29Z 2018-09-27T16:45:29Z 2020-09-28T09:15:13Z 2018-06-29 2018-08 Maternal protein restriction is associated with inducting of gene expression programs. Multiple stress-induced genes are known to be activated through eIF2α phosphorylation and induction of activating transcription factor 4 (ATF4), which also potentially play a role in autophagy related-genes expression. The present study investigates the mechanisms by which maternal protein restriction induce autophagy related-gene expression in the liver of offspring rats. In this study, pregnant Sprague-Dawley rats were fed either a control diet (18% energy from protein) or a low-protein diet (8.5% energy from protein) during gestation period followed by the control diet during lactation. Offspring consumed the same control post-weaning diet from postnatal day 21. Liver tissue was collected at postnatal day 38 from the offspring. The maternal low-protein diet significantly induced mRNA expression of the stress- response genes including Atf3 and Chop in liver of female offspring, but not in male. Moreover, mRNA expression of hepatic autophagy gene including LC3b, P62/SQSTM1, Beclin1, Atg3, Atg7, Atg5, and Atg10 was increased significantly only in the female offspring by gestational low-protein diet. The induction of gene expression was consistent with the activated eIF2a/ATF4 pathway in female offspring. Furthermore, we found the maternal low-protein diet induces ATF4 and C/EBP homology protein (CHOP) binding at the regions of specific autophagy-related genes in the liver of female offspring through differential cooperative regulation. Overall, our data demonstrated that hepatic autophagy gene expression could be induced by a maternal LP diet only in the female rat offspring. This transcriptional program involves in activated p- eIF2α/ATF4 pathway and cooperative regulation of transcription factors ATF4 and CHOP. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-08-01 The student, Mingzhu Cai, accepted the attached license on 2018-05-28 at 12:01. The student, Mingzhu Cai, submitted this Thesis for approval on 2018-05-28 at 12:10. This Thesis was approved for publication on 2018-06-29 at 09:16. DSpace SAF Submission Ingestion Package generated from Vireo submission #12592 on 2018-09-27 at 11:33:02 Made available in DSpace on 2018-09-27T16:45:29Z (GMT). No. of bitstreams: 2 CAI-THESIS-2018.pdf: 1884408 bytes, checksum: 1fa438c69c3bbe536330b0d82f389217 (MD5) LICENSE.txt: 4208 bytes, checksum: 49a8f8b134c10f3dc784ddcd230ef10e (MD5) Previous issue date: 2018-06-29 Embargo set by: Seth Robbins for item 107849 Lift date: 2020-09-27T16:45:39Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 107849 Lift date: 2020-09-27T16:47:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 107849 on 2020-09-28T09:15:13Z. application/pdf http://hdl.handle.net/2142/101749 en Copyright 2018 Mingzhu Cai Maternal low-protein diet, Autophagy, Gene, Transcription factor A maternal low-protein diet during gestation induces hepatic autophagy gene expression only in the female offspring Sprague-Dawley rats text text Food Science & Human Nutrition Food Science & Human Nutrition University of Illinois at Urbana-Champaign Thesis M.S.