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oai:www.ideals.illinois.edu:2142/105160 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Nair, Satish K. Nair, Satish K. van der Donk, Wilfred A. Gerlt, John A. Rienstra, Chad M. Cogan, Dillon P. 2019-08-23T20:44:38Z 2019-08-23T20:44:38Z 2021-08-24T09:15:38Z 2019-04-08 2019-05 Described herein is my thesis work towards structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, p-coumarate, and the remaining cases involve enzymes responsible for biosyntheses of complex natural products. Among the natural product cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide (PK-NRP) natural product biosynthesis is described. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01 The student, Dillon Cogan, accepted the attached license on 2019-04-03 at 18:53. The student, Dillon Cogan, submitted this Dissertation for approval on 2019-04-03 at 19:08. This Dissertation was approved for publication on 2019-04-08 at 16:47. DSpace SAF Submission Ingestion Package generated from Vireo submission #13487 on 2019-08-22 at 16:20:40 Made available in DSpace on 2019-08-23T20:44:38Z (GMT). No. of bitstreams: 2 COGAN-DISSERTATION-2019.pdf: 32735448 bytes, checksum: b645ad392d421bcceb37c1073332735a (MD5) LICENSE.txt: 4209 bytes, checksum: 52fd7dd5accac71456bceeb4b1910f0e (MD5) Previous issue date: 2019-04-08 Embargo set by: Seth Robbins for item 112279 Lift date: 2021-08-23T20:44:50Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 112279 Lift date: 2021-08-23T20:46:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 112279 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 112279 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 112279 on 2021-08-24T09:15:38Z. application/pdf http://hdl.handle.net/2142/105160 en Copyright 2019 Dillon Cogan biosynthesis natural product enzyme RiPP ribosomal peptide polyketide non-ribosomal DNA-binding thiopeptide lanthipeptide Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes text text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.