2026-09-22T00:38:48Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/105209 2023-07-11 col_2142_5131 com_2142_5130

The student, Stephan Lane, accepted the attached license on 2019-04-16 at 21:01. The student, Stephan Lane, submitted this Dissertation for approval on 2019-04-16 at 21:33. This Dissertation was approved for publication on 2019-04-18 at 10:50. DSpace SAF Submission Ingestion Package generated from Vireo submission #13694 on 2019-08-22 at 16:22:23 Made available in DSpace on 2019-08-23T20:47:30Z (GMT). No. of bitstreams: 3 LANE-DISSERTATION-2019.pdf: 3305614 bytes, checksum: 8750a1380b60b42e9d81d3338ac39b9a (MD5) Lane - Dissertation - Final.docx: 3899380 bytes, checksum: f21e8c9885e09ca9121f94a8fd298a67 (MD5) LICENSE.txt: 4209 bytes, checksum: 1005f2ab6d1ce33930eee4ba43f67952 (MD5) Previous issue date: 2019-04-18 Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 112330 on 2021-08-24T09:15:16Z. application/pdf http://hdl.handle.net/2142/105209 en Copyright 2019 Stephan Lane xylose, yeast, Saccharomyces cerevisiae, isobutanol, gadusol, sedoheptulose, lactic acid, metabolic engineering, cellobiose, cellulosic, biofuels Bioconversion of xylose into high-value products by engineered saccharomyces cerevisiae text text Jin, Yong-Su Stasiewicz, Matthew Cadwallader, Keith Rao, Christopher Lane, Stephan Thomas 2019-08-23T20:47:30Z 2019-08-23T20:47:30Z 2021-08-24T09:15:16Z 2019-04-18 2019-05 Significant efforts over the past few decades have focused on engineering the common brewer’s yeast Saccharomyces cerevisiae to consume xylose, the second-most abundant sugar in nature. Throughout these efforts, the goal has primarily been towards biofuels with ethanol as a target product. This research revealed that many aspects of xylose metabolism in yeast are unfavorable for production of ethanol and substantial efforts have been directed towards enhancing yeast’s limited ability to produce ethanol from xylose. With this narrow focus, many aspects of xylose metabolism have been overlooked which favor the production of non-ethanol compounds. I present here a literature review of all compounds which have been produced from xylose using engineered S. cerevisiae. Additionally, I will present personal research into developing engineering yeast strains capable of producing three compounds from xylose: isobutanol, sedoheptulose, and gadusol. Finally, I investigate the benefits of xylose metabolism towards lactic acid production and show that simultaneous co-fermentation of glucose and xylose leads to enhanced lactic acid yields. This work aims to highlight the benefits of xylose metabolism for many yeast metabolic engineering efforts and hopes to promote additional work into this fruitful area of research. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01 Food Science & Human Nutrition Food Science & Human Nutrition University of Illinois at Urbana-Champaign Dissertation Ph.D.