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Lu, Ting Sickle, Jordan 2020-03-02T22:38:56Z 2020-03-02T22:38:56Z 2022-03-03T10:15:27Z 2019-12-12 2019-12 Over the last two decades, synthetic biology has emerged as a critical toolset for genetic engineering. Synthetic gene circuits have been engineered for a variety of uses from functional modules to increasing biosynthetic yield. After decades of showing their potential in labs, synthetic circuits are about to make the leap into complex environments. Recent advances have shown that a mechanistic, coarse-grained approach can give fundamental insight into how synthetic circuits interact with their host cells, though how this model can be applied in complex environments remains an open question. In this thesis, I will discuss how an extension of this coarse-grained model can describe circuit behavior in complex environments through mechanistic modeling of chloramphenicol and nutrient stress. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-12-01 The student, Jordan Sickle, accepted the attached license on 2019-12-04 at 11:59. The student, Jordan Sickle, submitted this Thesis for approval on 2019-12-04 at 12:20. This Thesis was approved for publication on 2019-12-12 at 14:20. DSpace SAF Submission Ingestion Package generated from Vireo submission #14690 on 2020-02-28 at 17:37:53 Made available in DSpace on 2020-03-02T22:38:56Z (GMT). No. of bitstreams: 2 SICKLE-THESIS-2019.pdf: 3871832 bytes, checksum: e07b6d7ec523a3c9d28d802888a28d5e (MD5) LICENSE.txt: 4210 bytes, checksum: b585a1b761fd994b9ab13d5606a19281 (MD5) Previous issue date: 2019-12-12 Embargo set by: Seth Robbins for item 114028 Lift date: 2022-03-02T22:39:04Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 114028 on 2022-03-03T10:15:27Z. application/pdf http://hdl.handle.net/2142/106484 en Copyright 2019 Jordan Sickle Synthetic Biology Mathematical Modeling Towards extending coarse-grained circuit-host modeling to complex environments text text School of Molecular & Cell Bio Biophysics & Quant Biology University of Illinois at Urbana-Champaign Thesis M.S.