2026-09-21T16:08:52Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/109637 2023-07-11 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

Hergenrother, Paul J. Farmer, Rachel Elizabeth 2021-03-05T21:47:34Z 2021-03-05T21:47:34Z 2023-03-05T21:47:41Z 2020-12-08 2020-12 Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2022-12-01 The student, Rachel Farmer, accepted the attached license on 2020-12-07 at 16:50. The student, Rachel Farmer, submitted this Thesis for approval on 2020-12-07 at 16:50. This Thesis was approved for publication on 2020-12-08 at 11:23. DSpace SAF Submission Ingestion Package generated from Vireo submission #16085 on 2021-03-04 at 16:33:51 Made available in DSpace on 2021-03-05T21:47:34Z (GMT). No. of bitstreams: 2 FARMER-THESIS-2020.pdf: 1903637 bytes, checksum: 9a9d57c8c89c32f9cfdabdea23340814 (MD5) LICENSE.txt: 4210 bytes, checksum: 7cc027f83b8968e7a3c01f450d7d2e19 (MD5) Previous issue date: 2020-12-08 Embargo set by: Seth Robbins for item 117343 Lift date: 2023-03-05T21:47:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Author requested closed access (OA after 2yrs) in Vireo ETD system Limited application/pdf http://hdl.handle.net/2142/109637 en Copyright 2020 Rachel Farmer ARH3 PAR Screen High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors text Thesis Chemistry Chemistry University of Illinois at Urbana-Champaign Thesis M.S.