2026-09-21T07:45:26Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/110676 2023-07-11 col_2142_5131 col_2142_8888 com_2142_5130 com_2142_8887 com_2142_234

Boppart, Stephen A Yang, Lingxiao Lilian 2021-09-17T02:34:31Z 2021-09-17T02:34:31Z 2023-09-17T02:34:57Z 2021-04-16 2021-05 Needle biopsy (NB) is known as a standard clinical procedure for cancer diagnosis in humans and companion animals. There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber. Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01 The student, Lingxiao Lilian Yang, accepted the attached license on 2021-04-15 at 11:15. The student, Lingxiao Lilian Yang, submitted this Thesis for approval on 2021-04-15 at 11:28. This Thesis was approved for publication on 2021-04-16 at 14:48. DSpace SAF Submission Ingestion Package generated from Vireo submission #16346 on 2021-09-16 at 17:03:13 Made available in DSpace on 2021-09-17T02:34:31Z (GMT). No. of bitstreams: 3 YANG-THESIS-2021.pdf: 5362199 bytes, checksum: 0df445ad0f92880994994f507d5af47c (MD5) 1 - LilianThesis_ECEedited_LY_ECEv3.docx: 29188938 bytes, checksum: 6189d5eaa25b9b499fb94e4881091836 (MD5) LICENSE.txt: 4208 bytes, checksum: f882fad84b32a2ab8b4c9e54f5de6a4e (MD5) Previous issue date: 2021-04-16 Embargo set by: Seth Robbins for item 118519 Lift date: 2023-09-17T02:34:57Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system U of I Only application/pdf http://hdl.handle.net/2142/110676 en Copyright 2021 Lingxiao Lilian Yang Nonlinear optics biomedical imaging intraoperative imaging multiphoton microscopy cancer diagnosis Label-free multi-modal multiphoton imaging for real-time point-of-procedure assessment of needle biopsies text Thesis Electrical & Computer Eng Electrical & Computer Engr University of Illinois at Urbana-Champaign Thesis M.S.