2026-09-21T23:12:06Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/115420 2023-07-11 col_2142_5131 col_2142_5163 com_2142_5130 com_2142_5162 com_2142_234

Underhill, Gregory H 2022-05 application/pdf en text Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms The student, Hyeon Ryoo, accepted the attached license on 2022-04-21 at 12:02. The student, Hyeon Ryoo, submitted this Thesis for approval on 2022-04-21 at 12:11. This Thesis was approved for publication on 2022-04-25 at 16:24. DSpace SAF Submission Ingestion Package generated from Vireo submission #17879 on 2022-11-11 at 13:42:45 Spatially-defined quantitative interleukin 6 (IL-6) detection using polyethylene glycol microgel sensors (μGELISA) Ryoo, Hyeon 2022-04-25 Microgel Sensor Granular hydrogel IL-6 3D Cell Culture Polyethylene glycol Hepatic stellate cell Microwell Protein sensor ELISA Cells communicate with each other in multiple ways, with proteins as one of these signals. IL-6 is a pleiotropic cytokine involved in inflammation. IL-6 has a seemingly double-sided effect in liver pathologies, enhancing liver steatosis but also reducing liver injury. This ambiguous nature makes it imperative to find a research tool that can quantify local IL-6 concentrations in in vitro cell culture. By optimizing an ELISA system fitted into polyethylene glycol (PEG) microgels, we have developed an easy-to-use IL-6 sensor enabled for 3D cell culture systems. Using a confocal microscope, the microgels are able to detect IL-6 concentrations from 2.95 ng/mL and 33.56 ng/mL (middle 80% detection range). This concentration range fit well within the IL-6 release profile of small HSC aggregates, allowing us to quantify the exact IL-6 concentration starting at an average minimum distance of 14.68 μm from the aggregate. The μGELISA system could be adapted to proteins other than IL-6 by modifying the antibody sets used for capture and detection. Our μGELISA system could also be used to study cell-to-cell communication through protein signals and the effect these local concentrations have on each other, all within a closed-loop environment. Thesis eng https://hdl.handle.net/2142/115420 Copyright 2022 Hyeon Ryoo M.S. Thesis Bioengineering University of Illinois at Urbana-Champaign Bioengineering