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oai:www.ideals.illinois.edu:2142/115581 2023-07-11 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

Li, Xin Li, Xin Chen, Jie Smith-Bolton, Rachel Raetzman, Lori 2022-05 application/pdf en text Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01 The student, Alokananda Ray, accepted the attached license on 2022-04-19 at 12:16. The student, Alokananda Ray, submitted this Dissertation for approval on 2022-04-19 at 12:49. This Dissertation was approved for publication on 2022-04-21 at 11:52. DSpace SAF Submission Ingestion Package generated from Vireo submission #17801 on 2022-11-11 at 12:06:10 Mechanisms of regulation of sloppy-paired genes in the temporal patterning program of optic lobe medulla neuroblasts in drosophila Ray, Alokananda 2022-04-21 Temporal patterning optic lobe development neuroblasts Drosophila The generation of various neurons, each with specialized functional roles, from a small initial pool of neural stem cells presents a challenge for developing brains in higher animals. Temporal patterning, whereby neural stem cells specify distinct fates in their progeny in a birth-order dependent manner, is a crucial mechanism for achieving neural diversity and is conserved across species from fruit flies to mammals. However, detailed molecular mechanisms underlying temporal patterning are not entirely understood. In the work described in the following chapters, I have characterized the regulatory mechanisms controlling the expression of temporal patterning transcription factors Sloppy-paired 1 (Slp1) and Sloppy-paired 2 (Slp2) in optic lobe medulla neuroblasts in the model organism Drosophila melanogaster. I have demonstrated that at transcription, Slp1 and Slp2 are regulated by at least two cis-regulatory elements that function additively and drive expressions of these two genes in the pattern observed in medulla neuroblasts. I also showed that the Notch signaling pathway and the cell cycle contribute to the regulation of Slp1 and Slp2 protein expressions in this context. Together these results provide greater insight into how disparate cellular processes such as the cell cycle and signaling pathways such as Notch and temporal patterning interact to achieve precise developmental outcomes. Thesis eng https://hdl.handle.net/2142/115581 Copyright 2022 Alokananda Ray Ph.D. Dissertation Cell and Developmental Biology University of Illinois at Urbana-Champaign Cell & Developmental Biology