2026-09-22T16:40:55Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/115783 2023-07-11 col_2142_5131 col_2142_14737 com_2142_5130 com_2142_690 com_2142_397

Suski, Cory Maddox, Carol Van Bonn, William Weiner, Zachary 2022-05 application/pdf en text Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms The student, Vanessa DeShambo, accepted the attached license on 2022-04-25 at 19:39. The student, Vanessa DeShambo, submitted this Thesis for approval on 2022-04-25 at 19:46. This Thesis was approved for publication on 2022-04-29 at 12:47. DSpace SAF Submission Ingestion Package generated from Vireo submission #17956 on 2022-11-11 at 17:38:07 Surveillance for Burkholderia pseudomallei in aquaria DeShambo, Vanessa Jean 2022-04-29 Burkholderia pseudomallei microbiology aquarium aquaria surveillance fish tropical Melioidosis, a deadly disease of animals and humans, caused by the saprophytic bacteria Burkholderia pseudomallei, is an emerging disease. At present, there is little known about the prevalence of B. pseudomallei in tropical freshwater aquaria. Importation of animals, such as tropical fish, has been implicated in the introduction and spread of B. pseudomallei to new environments such as aquaria. There is a need to develop a robust and defensible sampling and detection plan, utilizing multiple sample types and techniques, to guide future investigations for the surveillance of B. pseudomallei in aquaria. Over two sampling periods, 101 samples were collected from tropical freshwater systems of the Shedd Aquarium and culture enriched for B. pseudomallei. A specific PCR targeting a 385 bp region of the recA gene was utilized to screen samples for the Burkholderia genus, and a real-time PCR targeting the Type Three Secretion System (TTS) was utilized in tandem to screen for B. pseudomallei. All samples were negative for both recA and TTS PCRs. My findings indicate that B. pseudomallei is not present in the sampling at the Shedd Aquarium, likely due to robust quarantine and disinfecting methods. The sampling plan presented here, coupled with a highly selective and sensitive real-time PCR, can be easily replicated for continued surveillance of B. pseudomallei or modified for other bacterial diseases. Thesis eng https://hdl.handle.net/2142/115783 Copyright 2022 Vanessa DeShambo M.S. Thesis Natural Res & Env Sciences University of Illinois at Urbana-Champaign Natural Res & Env Sci