2026-09-22T16:40:21Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/116026 2023-07-11 col_2142_5131 col_2142_5163 com_2142_5130 com_2142_5162 com_2142_234

Cunningham, Brian T Cunningham, Brian T Irudayaraj, Joseph Maria Kumar Nie, Shuming Smith, Andrew M. 2022-08 application/pdf en text Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01 The student, Congnyu Che, accepted the attached license on 2022-05-29 at 00:19. The student, Congnyu Che, submitted this Dissertation for approval on 2022-05-31 at 11:44. This Dissertation was approved for publication on 2022-06-22 at 15:54. DSpace SAF Submission Ingestion Package generated from Vireo submission #18047 on 2022-11-15 at 19:16:13 Multifunctional nanoparticle-coupled photonic resonator for rapid and digital biosensing Che, Congnyu 2022-06-22 photonic crystal magneto-plasmonic nanoparticle fast digital biosensing microRNA antibody detection multiplex detection The focus of this dissertation is to explore high-sensitivity, rapid, simple, cost-effective, and point-of-care (POC) compatible biomarker quantification approaches by multifunctional nanoparticle (NP)-coupled photonic crystal (PC) biosensors. Utilizing the enhanced absorption of the plasmonic NPs induced by the synergistic plasmonic-photonic coupling, each captured NP on the PC surface can be digitally counted with high signal-to-noise ratio, thus enabling near single-molecule detection of biomarkers. However, the applicability of PC-based approach to POC settings is limited by the mass transport of the analyte or NPs to the PC biosensor surface, which determines the sensitivity and response time of the assay. In this work, I explore two methods of overcoming this limitation: a self-powered microfluidic cartridge and the incorporation of magnetic actuation. First, the design and optimization of microfluidics are investigated to improve surface capture efficiency, followed by a demonstration of HIV antigen quantification assay with a single droplet test sample and femtomolar sensitivity. Next, magneto-plasmonic NPs are leveraged to offer digital microRNA detection and extraordinary testing speed by the dynamic control of nanoparticles. Finally, this work explores minimally invasive multiplexed antibody quantification using a portable imaging platform for POC diagnostics with short sample-to-answer time. Thesis eng https://hdl.handle.net/2142/116026 Copyright 2022 Congnyu Che Ph.D. Dissertation Bioengineering University of Illinois at Urbana-Champaign Bioengineering