2026-09-23T09:28:36Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/117769 2023-07-11 col_2142_5131 col_2142_11615 com_2142_5130 com_2142_9130 com_2142_8903

Rao, Christopher V Rao, Christopher V Leckband, Deborah E Shukla, Diwakar Jensen, Paul A 2022-12 application/pdf en text Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-04-12 without embargo terms The student, Sichong Pei, accepted the attached license on 2022-11-20 at 23:24. The student, Sichong Pei, submitted this Dissertation for approval on 2022-11-20 at 23:44. This Dissertation was approved for publication on 2022-11-28 at 15:57. DSpace SAF Submission Ingestion Package generated from Vireo submission #18604 on 2023-04-12 at 07:29:22 Biological methane conversion by methanotrophic bacteria Pei, Sichong 2022-11-28 Methanotroph Methane Oxidation In this dissertation, research works were presented on exploration of different aspects in biological methane conversion by methanotrophic bacteria using both experimental and computational techniques. First, robustness of methanotroph Methylococcus capsulatus Bath in raw natural gas was examined. Significant amount of hydrogen sulfide may exist in raw natural gas, and studies on its stress response toward toxicity of hydrogen sulfide remains unknown. An extensive investigation on viability of methanotroph in raw natural gas will improve its commercialization progress. Using next-generation sequencing technique and bioinformatic analysis, the transcriptomic change of M. capsulatus Bath toward toxicity of hydrogen sulfide was profiled at molecular level and it revealed unexpected observations. Although the presence of hydrogen sulfide slows the growth of M. capsulatus Bath, sulfide was shown to upregulate sulfide oxidation related enzymes, sulfide quinone reductase and persulfide dioxygenase as well as a novel lanthanide-dependent methanol dehydrogenase. Furthermore, efforts have been made on investigation of a feasible methane bioreactor. An inhouse bench-scale airlift column reactor was designed and constructed where methane conversion performance was studied. At last, the manuscript of an earlier cooperative publication on chemotaxis of Bacillus subtilis was included. Thesis eng https://hdl.handle.net/2142/117769 Copyright 2022 Sichong Pei Ph.D. Dissertation Chemical Engineering University of Illinois at Urbana-Champaign Chemical & Biomolecular Engr