2026-09-23T10:31:14Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/120568 2023-09-07 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Mera, Paola E Blanke, Steven Vanderpool, Carin Slauch, James 2023-05 application/pdf en text Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01 The student, Amanda Erlandson, accepted the attached license on 2023-04-27 at 16:13. The student, Amanda Erlandson, submitted this Dissertation for approval on 2023-04-27 at 16:36. This Dissertation was approved for publication on 2023-04-28 at 09:11. DSpace SAF Submission Ingestion Package generated from Vireo submission #19219 on 2023-09-01 at 17:22:01 The effect of antibiotic resistance on bacterial cell maintenance In gram-negative bacteria Erlandson, Amanda Lee 2023-04-28 Antibiotic Resistance Cellular Maintenance Cell Shape Antibiotic resistance has become a global concern, yet many of the mechanisms involved in antibiotic resistance remain poorly understood. Methods of antibiotic resistance are as diverse as antibiotics themselves, ranging from pump-driven mechanisms to eliminate a drug to host repair strategies to mitigate drug-induced damage. The research included in this thesis investigates the characteristics of two forms of antibiotic resistance found in bacteria: response to DNA-intercalators and efflux pumps. We demonstrate in vivo and in vitro that Ecm16 can render resistance against the DNA-intercalator echinomycin. Ecm16 belongs to a sub-class of UvrA-like proteins that are still poorly characterized. This work shows that, like UvrA, Ecm16 functionality requires two ATP-binding sites. Our collaborators have solved its crystal structure allowing a more in-depth assessment of its variation from other UvrA proteins. The second project focuses on physiological changes associated with the antibiotic efflux pump AcrAB-TolC. Although multi-drug efflux has been fairly well characterized, its consequences on cell fitness and development are still largely a mystery. My preliminary data revealed morphological changes in acrAB-tolC overexpression mutants in the presence of lignin-derived vanillate. Although recent research has hinted towards a connection between overexpression of AcrAB-TolC with changes in cell shape, this connection is still undefined. Collectively, the work presented in this thesis provide new insights to the various strategies that bacteria use to become antibiotic resistant. Thesis eng https://hdl.handle.net/2142/120568 Copyright 2023, Amanda Erlandson Ph.D. Dissertation Microbiology University of Illinois at Urbana-Champaign Microbiology