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oai:www.ideals.illinois.edu:2142/121357 2023-12-13 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Hatoum-Aslan, Asma Hatoum-Aslan, Asma Kuzminov, Andrei Mera, Paola E. Nair, Satish K. 2023-08 application/pdf en text Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01 The student, Lucy Chou Zheng, accepted the attached license on 2023-07-12 at 01:18. The student, Lucy Chou Zheng, submitted this Dissertation for approval on 2023-07-12 at 02:21. This Dissertation was approved for publication on 2023-07-13 at 11:25. DSpace SAF Submission Ingestion Package generated from Vireo submission #19642 on 2023-12-04 at 17:18:35 Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity Chou Zheng, Lucy 2023-07-13 Crispr-cas10 Type Iii-a Crispr-cas Cas10-csm Crispr-cas Degradosome, Staphylococci Staphylococcus Epidermidis Pnpase Rnase R Rnase J2 Nhi Anti-phage Defense. The overuse of antibiotics has led to a new wave of resistant bacterial pathogens insensitive to current antibiotics. Staphylococcus species are ubiquitous residents of human skin that can acquire and disseminate antibiotic resistance genes (ARGs) through horizontal gene transfer (HGT). Therefore, it is essential to understand cellular processes that naturally block HGT and prevent the transfer of ARGs. One such process is mediated by Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPRs) and their CRISPR-associated (Cas) proteins. CRISPR-Cas systems are a class of prokaryotic immune systems that use small CRISPR RNAs (crRNAs) as guides and Cas nucleases as weapons to detect and destroy mobile genetic elements, including plasmids and bacteriophages. The work in this thesis investigates the mechanism of CRISPR-Cas immunity using S. epidermidis RP62a, a commensal opportunistic pathogen, as the model organism. This research investigates and proves the central hypothesis that the Type III-A CRISPR-Cas system collaborates with housekeeping nucleases to mount a successful defense against genetic invaders. Text eng https://hdl.handle.net/2142/121357 Copyright 2023 Lucy Chou Zheng Ph.D. Dissertation Microbiology University of Illinois at Urbana-Champaign Microbiology