2026-09-21T10:37:09Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/122189 2024-03-02 col_2142_5131 col_2142_8859 com_2142_5130 com_2142_8858 com_2142_234

Kuehn, Seppe Golding, Ido Heath, Katy Maslov, Sergei 2023-12 application/pdf en text Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01 The student, Chandana Gopalakrishnappa, accepted the attached license on 2023-07-03 at 15:21. The student, Chandana Gopalakrishnappa, submitted this Dissertation for approval on 2023-07-03 at 23:51. This Dissertation was approved for publication on 2023-08-07 at 11:47. DSpace SAF Submission Ingestion Package generated from Vireo submission #19504 on 2024-03-01 at 13:47:33 Environmental modulators of algae-bacteria interactions at scale Gopalakrishnappa, Chandana Droplet Microfluidics High-throughput Microbial Ecology Phototroph-heterotroph Interactions High Dimensional Environments Statistical Modeling 2023-08-07 Photosynthetic microbes associated with non-photosynthetic, heterotrophic bacteria play a crucial role in global primary production, nutrient cycling, and industrial processes. Understanding these phototroph-heterotroph associations is therefore important but remains challenging because they reside in chemically complex aquatic and terrestrial environments. We do not understand how the myriad of environmental parameters, from nutrient availability to pH, impact interactions between phototrophs and their heterotrophic partners. Here, we leverage a massively parallel droplet microfluidic platform that enables us to interrogate algae-bacteria interactions in >100,000 communities across ~525 environmental conditions with varying pH, phosphorous availability. carbon availability and carbon source identity. By developing a statistical framework to dissect interactions in this complex dataset, we reveal that the dependence of algae-bacteria interactions on nutrient availability is strongly modulated by pH and buffering capacity. Furthermore, we show that the chemical identity of the available organic carbon source controls how pH, buffering capacity, and nutrient availability modulate algae-bacteria interactions. By leveraging a high-throughput platform, our study reveals the previously underappreciated role of pH in modulating phototroph-heterotroph interactions. Text eng https://hdl.handle.net/2142/122189 Copyright 2023 Chandana Gopalakrishnappa Ph.D. Dissertation Physics University of Illinois at Urbana-Champaign Physics