2026-09-21T20:24:37Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/127477 2026-02-03 col_2142_5131 col_2142_11615 com_2142_5130 com_2142_9130 com_2142_8903

application/pdf en text Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01 The student, Grace Rubino, accepted the attached license on 2024-11-29 at 09:52. The student, Grace Rubino, submitted this Thesis for approval on 2024-11-29 at 10:07. This Thesis was approved for publication on 2024-12-11 at 16:50. DSpace SAF Submission Ingestion Package generated from Vireo submission #21423 on 2025-03-28 at 14:55:27 Developing a medium-throughput collagen biomaterial model system Rubino, Grace Alexandra 2024-12-11 Harley, Brendan Bone Regeneration Collagen Scaffold Hydrogel Biomaterial eng The field of biomedical research has long recognized the need to develop higher order biomaterial model systems for improved disease characterization and translational therapeutic/material progress. There is, however, difficulty in developing these workflows at the scale of conventional two-dimensional cell culture screening systems while simultaneously approaching a level of complexity necessary to consider translation to in vivo animal models. Here, we describe a three-dimensional (3D), in vitro model system to investigate the impact of stromal cell migration from one microenvironment to another at a medium-throughput scale. Importantly, we demonstrate the ability of this workflow to be utilized as a screening tool for collagen-based biomaterial motifs of interest in promoting craniomaxillofacial bone defect repair. As potential next steps in utilization of this model, we probe questions underlying a dual modification of the mechanics and chemistry of a mesh collagen scaffold design for instructing bone regeneration, potential of a collagen scaffold to be drug loaded and quantifying a release profile, and lastly investigate collagen scaffolds in a new disease and cell context for understanding fibroblast mechanobiology. These serve as a preliminary set of materials designs for potential integration into the developed model system. Overall, we explore new routes of creating medium-throughput screening workflows and illuminate new material motifs that inform fundamental and translational biologic questions. 2024-12 Thesis https://hdl.handle.net/2142/127477 Copyright 2024 Grace A. Rubino Chemical & Biomolecular Engr Chemical Engineering University of Illinois at Urbana-Champaign M.S. Thesis