2026-09-22T20:11:11Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/129496 2026-02-05 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

application/pdf en text Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01 The student, Laura Suttenfield, accepted the attached license on 2025-03-01 at 23:02. The student, Laura Suttenfield, submitted this Dissertation for approval on 2025-03-01 at 23:15. This Dissertation was approved for publication on 2025-03-06 at 10:28. DSpace SAF Submission Ingestion Package generated from Vireo submission #21655 on 2025-10-19 at 19:14:19 Evolution of transmission mode in a temperate virus of Pseudomonas aeruginosa and its effects on the host Suttenfield, Laura Catherine 2025-03-06 Whitaker, Rachel J Whitaker, Rachel J Slauch, James M Kuzminov, Andrei Vanderpool, Carin lysogen virus transposable phage DMS3 Pseudomonas aeruginosa vertical transmission eng Bacteria are known for the infections they produce; that they are themselves infected is not often considered. This thesis considers the latent, lysogenic phase of a temperate virus DMS3 of the opportunistic pathogen Pseudomonas aeruginosa. In Chapter 1, I review the existing literature on how lysogeny contributes to viral fitness. In Chapter 2, we attempt to test this with deep sequencing of semi-random insertion sites. In Chapter 3, we find that spontaneous induction in lysogens causes divergent evolutionary resolutions. In Chapter 4, we take this approach to whole populations to test the way that infected and uninfected P. aeruginosa evolves in the presence of an antibiotic which can induce the lysogen. We find substantial evidence that this temperate virus impacts the evolution of its pathogen host. 2025-05 Thesis https://hdl.handle.net/2142/129496 Copyright 2025 Laura Suttenfield Microbiology Microbiology University of Illinois Urbana-Champaign Ph.D. Dissertation