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embryo Bagchi, Milan K. Katzenellenbogen, Benita S. Bagchi, Milan K. Kemper, Byron W. Freeman, Brian C. Raetzman, Lori T. Ramathal, Cyril Y. 2010-05-14T20:50:48Z 2010-05-14T20:50:48Z 2012-06-08T10:00:14Z 2010-05-14T20:50:48Z Embryo implantation into the endometrium is a complex biological process involving the integration of steroid hormone signaling, endometrial tissue remodeling and maternal- fetal communications. A successful pregnancy is the outcome of the timely integration of these events during the early stages of implantation. The involvement of ovarian steroid hormones, estrogen (E) and progesterone (P), acting through their cognate receptors, is essential for uterine functions during pregnancy. The molecular mechanisms that control the process of implantation are undergoing active exploration. Through our recent efforts, we identified the transcription factor, CCAAT Enhancer Binding Protein Beta (C/EBPb) as a prominent target of estrogen and progesterone signaling in the uterus. The development of a C/EBPb-null mouse model, which is infertile, presented us with an opportunity to analyze the role of this molecule in uterine function. We discovered that C/EBPb functions in two distinct manners: (i) by acting as a mediator of E-induced proliferation of the uterine epithelium and (ii) by controlling uterine stromal cell differentiation, a process known as decidualization, during pregnancy. My studies have delineated important mechanisms by which E regulates C/EBPb expression to induce DNA replication and prevent apoptosis of uterine epithelial cells during E-induced epithelial growth. In subsequent studies, I analyzed the role of C/EBPb in decidualization and uncovered a unique mechanism by which C/EBPb regulates the synthesis of a unique laminin-containing extracellular matrix (ECM) that supports stromal cell differentiation and embryo invasion. In order to better define the role of laminin in implantation, we developed a laminin gamma 1-conditional knockout mouse model. This is currently an area of ongoing investigation. The information gained from our analysis of C/EBPb function in the uterus provides new insights into the mechanisms of steroid hormone action during early pregnancy. Ultimately, our findings may aid in the understanding of dysregulation of hormone-controlled pathways that underlie early pregnancy loss and infertility in women. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-04-13T20:58:32Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Ramathal_Cyril.pdf: 73293117 bytes, checksum: 57cd7c7b0523113b64f23d054fdd9bbf (MD5) Made available in DSpace on 2010-05-14T20:50:48Z (GMT). No. of bitstreams: 4 Ramathal_Cyril.pdf: 73300825 bytes, checksum: 525e83c3bec27919da598f6035b3de79 (MD5) 1_Ramathal_Cyril.pdf: 73293117 bytes, checksum: 57cd7c7b0523113b64f23d054fdd9bbf (MD5) 2_Ramathal_Cyril.pdf: 73299929 bytes, checksum: 5f9491197da494b72755cff1d3afb08f (MD5) license.txt: 4063 bytes, checksum: 295c0c4302775efb5167a7e136275d5b (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-05-14T20:52:46Z Item is restricted until 2012-05-14T20:52:43Z Item marked as restricted to the 'Administrator' Group (id=1) by Sarah Shreeves (sshreeve@illinois.edu) on 2010-07-07T23:34:00Z Item is restricted until 2012-06-07T23:34:00Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-06-08T10:00:14Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Cell and Developmental Biology (ID: 702) No. of bitstreams: 5 2_Ramathal_Cyril.pdf.txt: 243412 bytes, checksum: c3e1a44e01db98b7cbe94ca5393c5727 (MD5) Ramathal_Cyril.pdf: 73300825 bytes, checksum: 525e83c3bec27919da598f6035b3de79 (MD5) 1_Ramathal_Cyril.pdf: 73293117 bytes, checksum: 57cd7c7b0523113b64f23d054fdd9bbf (MD5) 2_Ramathal_Cyril.pdf: 73299929 bytes, checksum: 5f9491197da494b72755cff1d3afb08f (MD5) license.txt: 4063 bytes, checksum: 295c0c4302775efb5167a7e136275d5b (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-06-08T10:00:14Z http://hdl.handle.net/2142/15569 en Chapter 1, copyrighted by Thieme Medical Publishers Chapter 2, copyrighted by American Society of Microbiology. Implantation Infertility decidualization uterus endometrium estrogen progesterone Extracellular Matrix (ECM) Analysis of the role of the transcription factor C/EBPβ in controlling uterine functions during early pregnancy 2010-5 Cell & Developmental Biology 1584 Cell and Developmental Biology 4094 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Cell&Develpmntl Biol -UIUC 10KS4094PHD