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Kent, Angela D. Chang, Yu-Rui 2010-05-18T18:55:36Z 2010-05-18T18:55:36Z 2012-05-19T10:00:15Z 2010-05-18T18:55:36Z 2010-5 Strong correlations between bacterial communities and algal seasonal succession have been previously observed. In aquatic systems, dissolved organic carbon derived by algae is an important resource for bacteria. Light and phosphorus availability are two factors that influence biomass and abundance of algae, and the changes will be reflected in the bacterial portion of the microbial community. Glycolate is an algal-specific exudate produced under excess light conditions. Glycolate uptake by bacteria has been shown to correlate with algal primary productivity. Bacterial populations that utilize glycolate possess the gene, glycolate oxidase subunit D (glcD). This gene was used as a marker to identify changes in specific bacterial populations that respond to algal exudates. In this study, development of periphyton biofilms in an experimental stream system was monitored across different light and phosphorus levels. Samples were collected every two days for community and chemistry analyses. Bacterial communities were monitored using DNA fingerprinting techniques based on ribosomal RNA genes and the glcD gene. We demonstrated that bacterial community composition changed significantly over the course of biofilm development, and light and phosphorus availability contributed to those differences in community composition. Our results suggest that a strong coupling between carbon flow and bacterial community composition. These results increase our understanding of the ecological drivers that impact benthic biofilm communities that carry out transformation of nutrients in streams. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-04-13T21:36:52Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 5 Chang_Yu-rui.docx: 3083711 bytes, checksum: b908f92f8cf086ef1ec7291afa46842f (MD5) Chang_Yu-rui.pdf: 2437846 bytes, checksum: 9b228df94322ec666a9c5fd114486185 (MD5) Chang_Yu-rui.pdf: 1199154 bytes, checksum: d6bb7412a639ed5e1434cc374ea74ff1 (MD5) Chang_Yu-rui.pdf: 1199878 bytes, checksum: 0269605873fc2601bd0e4497349679c2 (MD5) Chang_Yu-rui.pdf: 2436414 bytes, checksum: eaecfbd17dcc91d6b8a8fd0f7ad971a2 (MD5) Made available in DSpace on 2010-05-18T18:55:36Z (GMT). No. of bitstreams: 7 Chang_Yu-rui.docx: 3113630 bytes, checksum: 32429bfca4816ddaf85aec029851d78b (MD5) Chang_Yu-rui.pdf: 2436414 bytes, checksum: eaecfbd17dcc91d6b8a8fd0f7ad971a2 (MD5) 1_Chang_Yu-rui.pdf: 2437846 bytes, checksum: 9b228df94322ec666a9c5fd114486185 (MD5) 2_Chang_Yu-rui.pdf: 1199154 bytes, checksum: d6bb7412a639ed5e1434cc374ea74ff1 (MD5) 3_Chang_Yu-rui.pdf: 1199878 bytes, checksum: 0269605873fc2601bd0e4497349679c2 (MD5) 4_Chang_Yu-rui.pdf: 2464534 bytes, checksum: 32f5080625e3dbc06047489b6ead9f98 (MD5) license.txt: 4058 bytes, checksum: 742df0864f7263ec4ae3db19ce373cfb (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-05-18T18:56:43Z Item is restricted until 2012-05-18T18:56:42Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:15Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Natural Resources and Environmental Sciences (ID: 671) No. of bitstreams: 8 4_Chang_Yu-rui.pdf.txt: 93696 bytes, checksum: 0db5e31874ffea9a7863de26aed355a9 (MD5) Chang_Yu-rui.docx: 3113630 bytes, checksum: 32429bfca4816ddaf85aec029851d78b (MD5) Chang_Yu-rui.pdf: 2436414 bytes, checksum: eaecfbd17dcc91d6b8a8fd0f7ad971a2 (MD5) 1_Chang_Yu-rui.pdf: 2437846 bytes, checksum: 9b228df94322ec666a9c5fd114486185 (MD5) 2_Chang_Yu-rui.pdf: 1199154 bytes, checksum: d6bb7412a639ed5e1434cc374ea74ff1 (MD5) 3_Chang_Yu-rui.pdf: 1199878 bytes, checksum: 0269605873fc2601bd0e4497349679c2 (MD5) 4_Chang_Yu-rui.pdf: 2464534 bytes, checksum: 32f5080625e3dbc06047489b6ead9f98 (MD5) license.txt: 4058 bytes, checksum: 742df0864f7263ec4ae3db19ce373cfb (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-05-19T10:00:15Z http://hdl.handle.net/2142/15997 en Copyright 2010 Yu-rui Chang biofilm periphyton light and nutrient hypothesis light and phosphorus ratio algal exudate glycolate glycolate oxidase glcD gene microbial community automated ribosomal intergenic spacer analysis (ARISA) Terminal Restriction Fragment Length Polymorphism (T-RFLP) 16S ribosomal RNA gene Bacterial community composition in stream biofilms is influenced by algal response to varying light and phosphorus ratios Natural Res & Env Sci 1875 Natural Res & Env Sciences 0190 University of Illinois at Urbana-Champaign Thesis M.S. MS:Nat Res & Envrn Sci -UIUC 10KS0190MS