2026-09-19T09:21:47Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/16789 2023-07-10 col_2142_5131 col_2142_16308 com_2142_5130 com_2142_16307 com_2142_8913 com_2142_8903

Robinson, Gene E. Naeger, Nicholas L. 2010-08-20T17:57:53Z 2010-08-20T17:57:53Z 2010-08-20T17:57:53Z 2010-08 Honey bees feed on flowers, many of which produce nectar during certain restricted, but consistent, time windows in the day. Previous research has shown that honey bees have the ability to form distinct spatiotemporal memories that allow them to return repeatedly to different attractive food sources at different times of day. As a first step toward understanding the molecular basis of this striking flower fidelity, this study was designed to determine whether distinct spatiotemporal memories of a food source are associated with distinct patterns of gene expression in the brain. Different groups of foragers from the same colony were trained to collect sucrose solution from an artificial feeder at one of two different times of day, one in the morning and the other in the afternoon. Bees from both training groups were collected at both training times, thus yielding a set of bees that was undergoing stereotypical food anticipatory behavior and another set that was inactive for each collection. Microarray analysis revealed that hundreds of genes were differentially expressed in the brain based on either time of day or activity state. A subset of these genes showed unique patterns of expression for each of the spatiotemporal foraging memories. Surprisingly, some of the genes involved in the regulation of circadian rhythms, such as period and cryptochrome, appear to change in expression with activity state rather than time of day. Overall, these results indicate that distinct spatiotemporal foraging memories in honey bees are associated with distinct neurogenomic states involving genes that regulate temporal activity and genes associated with appetitive behavior. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-07-21T19:15:15Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Naeger Nicholas.pdf: 4241948 bytes, checksum: b2c63738f7c92bae445aeebc80a0f57b (MD5) Made available in DSpace on 2010-08-20T17:57:53Z (GMT). No. of bitstreams: 5 Naeger Nicholasv2.pdf: 1105372 bytes, checksum: 8c21a4a053d5fcaaa8cb5b7158e35580 (MD5) Naeger Nicholas.pdf: 4241948 bytes, checksum: b2c63738f7c92bae445aeebc80a0f57b (MD5) 1_Naeger Nicholas.pdf: 4241948 bytes, checksum: b2c63738f7c92bae445aeebc80a0f57b (MD5) 2_Naeger Nicholas.pdf: 1105371 bytes, checksum: 455605154a4f5d1ab66eb30cbf2e6852 (MD5) license.txt: 4065 bytes, checksum: 3d23e168815c7b40912a64f06f59205b (MD5) http://hdl.handle.net/2142/16789 en Copyright 2010 Nicholas L. Naeger Honey bee Apis mellifera microarray appetitive behavior spatiotemporal memories circadian Spatiotemporal memories in honey bees: microarray analysis of time-trained foragers Entomology 1361 Entomology 0324 University of Illinois at Urbana-Champaign Thesis M.S. MS:Entomology -UIUC 10KS0324MS