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Insana, Michael F. Xu, Jing 2010-08-31T20:02:23Z 2010-08-31T20:02:23Z 2012-09-07T16:43:32Z 2010-08-31T20:02:23Z 2010-08 In this work, we explored a thick in vitro 3-D cell culture model with layered structure and applied transforming growth factor-beta1 (TGF-β1) on the top of the 3-D culture to induce the conversion from normal fibroblasts to myofibroblasts and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. In summary, we present here a handy experimental and computational tool for researches on tumor signaling pathways, and provide some guidance for studies on diffusion of protein factors or drugs in 3-D tissue environment. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-06-24T18:08:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 3 Xu_Jing.pdf.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) Xu_Jing.docx: 5317697 bytes, checksum: 072c566679ea0707ba4e8295d35e9141 (MD5) Xu_Jing.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) Made available in DSpace on 2010-08-31T20:02:23Z (GMT). No. of bitstreams: 6 Xu_Jing.pdf: 2158127 bytes, checksum: 6c7ddcd0e38cbf3865316cf2e97f3e50 (MD5) 1_Xu_Jing.pdf: 2158127 bytes, checksum: 6c7ddcd0e38cbf3865316cf2e97f3e50 (MD5) 2_Xu_Jing.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) Xu_Jing.pdf.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) 3_Xu_Jing.pdf: 2161714 bytes, checksum: 70e4e5b7ee51a6d35d262e65cea3cd66 (MD5) license.txt: 4056 bytes, checksum: 621479f0a94a0d07d8706dac8d271339 (MD5) Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:04:47Z Item is restricted until 2012-08-31T20:04:44Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:32Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Theses and Dissertations - Bioengineering (ID: 225) No. of bitstreams: 7 Xu_Jing.pdf: 2158127 bytes, checksum: 6c7ddcd0e38cbf3865316cf2e97f3e50 (MD5) 1_Xu_Jing.pdf: 2158127 bytes, checksum: 6c7ddcd0e38cbf3865316cf2e97f3e50 (MD5) 2_Xu_Jing.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) Xu_Jing.pdf.pdf: 16129084 bytes, checksum: b4da50f8caa619b94c639b4317b2a223 (MD5) 3_Xu_Jing.pdf: 2161714 bytes, checksum: 70e4e5b7ee51a6d35d262e65cea3cd66 (MD5) license.txt: 4056 bytes, checksum: 621479f0a94a0d07d8706dac8d271339 (MD5) 3_Xu_Jing.pdf.txt: 89422 bytes, checksum: ca2b7fecdacfaec736056040ad1b4182 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:32Z http://hdl.handle.net/2142/16963 en Copyright 2010 Jing Xu myofibroblasts transforming growth factor-beta1 alpha-smooth muscle actin diffusion confocal microscopy imaging immunofluorescence staining Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system Thesis Bioengineering 1343 Bioengineering 0408 University of Illinois at Urbana-Champaign M.S. MS:Bioengineering - UIUC 10KS0408MS