2026-09-21T09:20:09Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/17028 2023-07-10 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Hergenrother, Paul J. Hergenrother, Paul J. Cunningham, Brian T. Shapiro, David J. Morrissey, James H. Heeres, James T. 2010-08-31T20:29:49Z 2010-08-31T20:29:49Z 2012-09-07T16:43:37Z 2010-08-31T20:29:49Z 2010-08 High-throughput screening (HTS) has played an integral role in the discovery of lead molecules that eventually are transformed into the therapies of tomorrow. Contributing towards the success of any screening campaign is the target choice, chemical library size and diversity, as well as the technology utilized in screening. Photonic crystal (PC) biosensors are a relatively new technology whose potential is only beginning to be realized. PC biosensors are a “labelfree” technology compatible with standard high-throughput techniques. Macromolecular interactions including protein—small molecule, protein—nucleic acid, protein—protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein—nucleic acid interaction and a simplified protein— protein interaction platform. The protein—nucleic acid interaction targeted is the apoptosis inducing factor (AIF)—DNA interaction, which is critical for the mediation of caspaseindependent cell death in neurodegenerative disease and stroke. Protein—protein interactions were targeted by modifying the PC biosensor surface for detection of the His6 tag, which is widely employed in protein purification. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-05-19T13:33:51Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Heeres_James.pdf: 34917397 bytes, checksum: 300d8d43356d5ee9f3b1b952a4722367 (MD5) Made available in DSpace on 2010-08-31T20:29:49Z (GMT). No. of bitstreams: 3 Heeres_James.pdf: 34917397 bytes, checksum: 300d8d43356d5ee9f3b1b952a4722367 (MD5) 1_Heeres_James.pdf: 34917422 bytes, checksum: 0cac108c4ca55e3ab42d04a76c9c183e (MD5) license.txt: 4062 bytes, checksum: 76f135a9cb0a21f986c13c63dc4cd79c (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:32:51Z Item is restricted until 2012-08-31T20:32:49Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:37Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Biochemistry (ID: 679) No. of bitstreams: 4 Heeres_James.pdf: 34917397 bytes, checksum: 300d8d43356d5ee9f3b1b952a4722367 (MD5) 1_Heeres_James.pdf: 34917422 bytes, checksum: 0cac108c4ca55e3ab42d04a76c9c183e (MD5) license.txt: 4062 bytes, checksum: 76f135a9cb0a21f986c13c63dc4cd79c (MD5) 1_Heeres_James.pdf.txt: 336136 bytes, checksum: c96995b024eb2215976ca301a60ff9d5 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:37Z http://hdl.handle.net/2142/17028 en Copyright 2010 James T. Heeres Photonic Crystals Apoptosis Inducing Factor apoptosis inducing factor (AIF) Protein-DNA Interactions Protein-Protein Interactions High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor Biochemistry 1438 Biochemistry 0318 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Biochemistry -UIUC 10KS0318PHD