2026-09-20T17:20:15Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/17057 2023-07-10 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

Newmark, Phillip A. Belmont, Andrew S. Newmark, Phillip A. Brieher, William M. Wang, Fei Yao, Humphrey H-C. Wang, Yuying 2010-08-31T20:30:51Z 2010-08-31T20:30:51Z 2012-09-07T16:43:39Z 2010-08-31T20:30:51Z 2010-08 Our understanding of molecular mechanisms involved in germ cell development is limited to classical genetic model organisms and would benefit from studying additional animal models. Freshwater planarian provides a unique system for studying germ cell development: these animals appear to form their germline post-embryonically from neoblasts, the totipotent stem cells of the species. In this study, we aim to systematically investigate germ cell development in planarian species Schmidtea mediterranea. We first identified a S. mediterranea homolog of the conserved early germ cell marker and germ granule component nanos and showed that it is required for planarian germline development, maintenance, and regeneration. Then we took a microarray analysis approach to identify planarian germ cell-specific transcripts and validated their expression in planarian germline by whole-mount in situ hybridization (WISH). Finally, we carried out a RNA interference screen to analyze functions of these germline-specific genes in planarian germ cell development. We successfully identified a list of genes, encoding both conserved and novel factors, which play important roles regulating various stages of planarian germ cell differentiation. This work lays the groundwork for future studies using planarian as a model system to investigate molecular mechanisms underlying germ cell development. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-07-02T20:41:30Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Wang_Yuying.doc: 60038656 bytes, checksum: 88072b8a0a4a024e72a941600072deed (MD5) Wang_Yuying.pdf: 49352103 bytes, checksum: ef69770b5f72bd841f77f9386af93a7c (MD5) Made available in DSpace on 2010-08-31T20:30:51Z (GMT). No. of bitstreams: 3 Wang_Yuying.doc: 60038656 bytes, checksum: 88072b8a0a4a024e72a941600072deed (MD5) Wang_Yuying.pdf: 49352103 bytes, checksum: ef69770b5f72bd841f77f9386af93a7c (MD5) license.txt: 4059 bytes, checksum: e9659377ce6bf48f63287850b0d6347d (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:32:55Z Item is restricted until 2012-08-31T20:32:49Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:39Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Cell and Developmental Biology (ID: 702) No. of bitstreams: 4 Wang_Yuying.doc: 60038656 bytes, checksum: 88072b8a0a4a024e72a941600072deed (MD5) Wang_Yuying.pdf: 49352103 bytes, checksum: ef69770b5f72bd841f77f9386af93a7c (MD5) license.txt: 4059 bytes, checksum: e9659377ce6bf48f63287850b0d6347d (MD5) Wang_Yuying.pdf.txt: 254250 bytes, checksum: cd428fda50f0927228de0a972713ff75 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:39Z http://hdl.handle.net/2142/17057 en Copyright 2010 Yuying Wang planarian germ cell development nanos RNAi screen Dissecting the molecular mechanisms of germ cell development in the planarian Schmidtea mediterranea Cell & Developmental Biology 1584 Cell and Developmental Biology 4094 University of Illinois at Urbana-Champaign Dissertation Ph.D. PHD:Cell&Develpmntl Biol -UIUC 10KS4094PHD