2026-09-20T07:53:43Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/17064 2023-07-10 col_2142_5131 col_2142_14770 com_2142_5130 com_2142_3518 com_2142_234

Copyright 2010 Sean K. Poust Polymer vesicles Zilles, Julie L. Poust, Sean K. 2010-08-31T20:31:10Z 2010-08-31T20:31:10Z 2012-09-07T16:43:40Z 2010-08-31T20:31:10Z 2010-08 This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-07-21T22:37:40Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) Made available in DSpace on 2010-08-31T20:31:10Z (GMT). No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5) Item marked as restricted to the 'Administrator' Group (id=1) by William Ingram (wingram2@illinois.edu) on 2010-08-31T20:32:56Z Item is restricted until 2012-08-31T20:32:49Z Item reinstated by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z Item was in collections: University of Illinois Dissertations and Theses (ID: 204) Dissertations and Theses - Civil and Environmental Engineering (ID: 672) No. of bitstreams: 3 Poust_Sean.pdf: 1845282 bytes, checksum: 2de59c765454fc4f52370dcd94ad9fd4 (MD5) Thesis Draft Repaired.docx: 20117161 bytes, checksum: 7fb29d315c36725b7065d3251835c42a (MD5) license.txt: 4057 bytes, checksum: 5fe31469e97532d29b845bb5dd48e650 (MD5) Item released from any restrictions by Sarah Shreeves (sshreeve@illinois.edu) on 2012-09-07T16:43:40Z http://hdl.handle.net/2142/17064 en Perchlorate treatment Synthetic biology Water treatment Engineered Vesicles for Perchlorate Degradation Civil & Environmental Eng 1251 Environ Engr in Civil Engr 0231 University of Illinois at Urbana-Champaign Thesis M.S. MS:Env Engr Civil Engr -UIUC 10KS0231MS