2026-09-22T06:05:36Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/18300 2023-07-10 col_2142_16405 col_2142_5131 com_2142_16404 com_2142_14833 com_2142_5130

Stewart, Allison A. Durgam, Sushmitha S. 2011-01-14T22:45:26Z 2011-01-14T22:45:26Z 2011-01-14T22:45:26Z This study was performed to determine the effects of fibroblast growth factor-2 (FGF-2) on monolayer expansion of equine tendon- and bone marrow-derived cells prior to culture with autogenous acellular tendon matrix and insulin-like growth factor-I (IGF-I). Progenitor cells were isolated from six young adult horses, expanded in monolayers with FGF-2, and cultured with autogenous acellular pulverized tendon and IGF-I for seven days. Initial cell isolation and subsequent monolayer proliferation were assessed. In the cell: pulverized tendon cultures, cell viability, expression of collagen types I and II, and cartilage oligomeric matrix protein (COMP) mRNAs, collagen and glycosaminoglycans (GAG) syntheses were assessed. Tendon-derived cells proliferated significantly more rapidly in the initial monolayer expansion cultures in comparison to bone marrow-derived cells. Further, monolayer expansion with FGF-2 significantly increased the cell numbers of tendon-derived cells. Expression of collagen type I, collagen type III and COMP mRNAs was higher in tendon-derived cell groups than bone marrow-derived cell groups. However, IGF-I supplementation significantly increased collagen type I and type III mRNA expression in only the bone marrow-derived cell groups. IGF-I supplementation significantly increased collagen synthesis of bone marrow-derived cells. Monolayer expansion with FGF-2 followed by IGF-I supplementation significantly increased proteoglycan synthesis in tendon-derived cells. In summary, tendon-derived cell cultures generated more cells and showed increased matrix synthesis following monolayer expansion with FGF-2 when compared to bone marrow-derived cells. In vivo experiments using FGF-2 expanded tendon-derived cells are warranted to evaluate the effects on tendon healing. Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2010-12-08T16:39:39Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Durgam_Sushmitha.doc: 4170752 bytes, checksum: db2e8e8ffaeff9dc77c3a6b35565b819 (MD5) Durgam_Sushmitha.pdf: 3726834 bytes, checksum: f1697186a984df6e96f92fcdfe941496 (MD5) Made available in DSpace on 2011-01-14T22:45:26Z (GMT). No. of bitstreams: 3 Durgam_Sushmitha.pdf: 3726630 bytes, checksum: d004be16a982ce588501db02dfad6673 (MD5) license.txt: 4066 bytes, checksum: 647fd7d06da9699fe1d934e19eb22ed5 (MD5) Durgam_Sushmitha.doc: 4170752 bytes, checksum: db2e8e8ffaeff9dc77c3a6b35565b819 (MD5) http://hdl.handle.net/2142/18300 en Copyright 2010 Sushmitha S. Durgam Tendon healing Progenitor cells In vitro comparison of equine tendon- and bone marrow-derived cells expanded with FGF-2 prior to culturing with tendon matrix and IGF-I 2010-12 Vet Clinical Medicine 1598 VMS-Veterinary Clinical Medcne 0359 University of Illinois at Urbana-Champaign Thesis M.S. MS:VMS-Vet Clinical Med -UIUC 10KS0359MS