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oai:www.ideals.illinois.edu:2142/18940 2023-07-10 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Kaplan, Samuel Maloy, Stanley R. Moore, Mark David 2011-05-07T11:52:03Z 2011-05-07T11:52:03Z 10000-01-01 1994 "Intrinsic high-level resistance (HLR) to tellurite (TeO$\sb3\sp{2-}),$ selenite (SeO$\sb3\sp{2-}),$ and at least fifteen other chalcogenic oxides and oxyanions has been demonstrated in the facultative photoheterotrophic bacterium, Rhodobacter (Rba.) sphaeroides. Detailed analyses have shown that HLR to at least one class of these oxyanions, the ""tellurite class"" (e.g., $\rm TeO\sb4\sp{2-},\ TeO\sb3\sp{2-},\ SeO\sb4\sp{2-},\ SeO\sb3\sp{2-},$ and $\rm Rh\sb2O\sb3),$ occurred via a metalloid oxyanion reductase (MORase), and resulted in elemental deposition in the cytoplasmic membrane, and concomitant H$\sb2$ evolution during photoheterotrophic growth in the presence of these oxyanions. Based on the analysis of TnphoA-derived mutants, the periplasmic-localized dimethyl sulfoxide/trimethylamine-N-oxide reductase (which has been isolated and purified to homogeneity) is not involved in the reduction of these oxyanions. Examination of other Rba. sphaeroides mutants, however, has determined the obligate requirement for an intact CO$\sb2$ fixation pathway and the presence of a functional photosynthetic electron transport chain to effect HLR to TeO$\sb3\sp{2-}$-class oxyanions under photosynthetic growth, and functional cytochromes $b,\ c\sb1$ and $c\sb2$ to facilitate HLR under aerobic growth conditions." Other members of the Rhodospirillaceae, including members of the $\alpha$-2 and $\alpha$-3 subgroups, were also shown to effect the reduction of many of these compounds, although genera from the $\alpha$-1, $\beta$-1, and $\gamma$-3 subgroups did not. Fifty strains representing six species of purple non-sulfur bacteria were examined for intrinsic HLR to TeO$\sb3\sp{2-}.$ These species were further characterized by total genomic DNA restriction pattern analysis using low-frequency cleavage restriction endonucleases and transverse alternating-field gel electro-phoresis. Genomic schizotypes were obtained for wild-type strains of Rba. sphaeroides and related Proteobacteria. Analysis of these macrorestriction pattern polymorphisms enabled a rapid differentiation of closely-related strains and permitted an estimation of the minimum genome size of each strain. The genetic relationship of these bacteria has been established using maximum parsimony analyses. Evidence is presented for the existence of considerable genetic diversity and genome plasticity among seemingly similar wild-type strains. It has been demonstrated using readily-identifiable DNA macrorestriction fragments that not all strains currently identified as Rba. sphaeroides contain two chromosomes. Genetic schizotyping of strains contained in international culture depositories revealed that not all Rba. sphaeroides strains currently bearing the type strain designation are identical. Based on these results, a major rearrangement of the genus Rhodobacter is proposed. Made available in DSpace on 2011-05-07T11:52:03Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503276.pdf: 8014049 bytes, checksum: f8e05b4e7511a914e9ac6ef221cf93f9 (MD5) Previous issue date: 1994 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:33:34Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:12:26-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9503276 (UMI)AAI9503276 http://hdl.handle.net/2142/18940 eng Copyright 1994 Moore, Mark David Biology, Molecular Biology, Genetics Biology, Microbiology Genetic relatedness of the family Rhodospirillaceae: Genome plasticity, chromosome heterogeneity, and chalcogenic oxyanion reductase activity in Rhodobacter sphaeroides text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.