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oai:www.ideals.illinois.edu:2142/19090 2023-07-10 col_2142_5131 col_2142_14797 com_2142_5130 com_2142_14796 com_2142_397

Milner, J.A. 1994 Hwang, Kyung Hee 2011-05-07T11:56:38Z 2011-05-07T11:56:38Z 10000-01-01 Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\rm\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation. Made available in DSpace on 2011-05-07T11:56:38Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503220.pdf: 6445595 bytes, checksum: 398f8fefef81bcd5c7dd03e7ccb8bf46 (MD5) Previous issue date: 1994 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:34:35Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:13:17-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9503220 (UMI)AAI9503220 http://hdl.handle.net/2142/19090 eng Copyright 1994 Hwang, Kyung Hee Biology, Molecular Biology, Cell Agriculture, Animal Culture and Nutrition Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells text Nutritional Sciences Nutritional Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.