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oai:www.ideals.illinois.edu:2142/19223 2023-07-10 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Bankaitis, Vytas A. Skinner, Henry Bradford 2011-05-07T12:00:46Z 2011-05-07T12:00:46Z 10000-01-01 1995 The Saccharomyces cerevisiae phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein (SEC14p) is required for Golgi function in vivo. Mutations that specifically result in the inactivation of the CDP-choline pathway for PC biosynthesis bypass the requirement for SEC14p. The in vivo consequences of SEC14p dysfunction have been shown to result in the CDP-choline pathway driven increase in Golgi PC content. Overproduction of the SEC14p is found to manifest a decrease in the cellular PC content due to an inhibition of the CDP-choline pathway. This phenotype is reversed in cells that also overproduce the yeast cholinephosphatecytidylyltransferase (CCTase). The SEC14p is shown to act as a negative regulator of the CDP-choline pathway through the ligand-modulated SEC14p dependent inhibition of CCTase. PC bound SEC14p is identified as likely the active agent in the inhibition of CCTase. SEC14p is further shown to be conserved throughout the yeasts. A gene for the Schizosaccharomyces pombe SEC14p was cloned and its nucleotide sequence determined. The S. pombe SEC14p is highly homologous to the S. cerevisiae SEC14p and functionally replaces the S. cerevisiae SEC14p in vivo. Made available in DSpace on 2011-05-07T12:00:46Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9522178.pdf: 3582327 bytes, checksum: b171d46cf618377553c8564ed3a07fd7 (MD5) Previous issue date: 1995 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:29Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:14:00-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9522178 (UMI)AAI9522178 http://hdl.handle.net/2142/19223 eng Copyright 1995 Skinner, Henry Bradford Biology, Molecular Biology, Cell The Saccharomyces cerevisiae phosphatidylinositol/phosphatidylcholine transfer protein functions as a negative feedback regulator of the CDP-choline pathway text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.