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Swiader, John M. Al-Redhaiman, Khalid Nasser 2011-05-07T12:02:18Z 2011-05-07T12:02:18Z 10000-01-01 1996 The research in this thesis is made up of three related experiments, each concerned with nitrate (NO$\sb{3-})$ accumulation and reduction in hydroponically-grown lettuce (Lactuca sativa L.), and its relationship to various aspects of N supply, including solution NO$\sb{3-}$ concentration, the nitrate:ammonium solution ratio, and the interaction between solution flow rate and NO$\sb{3-}$ fertilization level. In the first experiment, significant effects from both solution NO$\sb{3-}$ rate and genotype influenced tissue NO$\sb{3-}$ concentrations. A sustained concentration of 1.0 mM NO$\sb{3-}$ in solution was adequate to support lettuce growth, as there was very little response in either fresh or dry shoot weight with increasing NO$\sb{3-}$ supply up to 5.0 and 15.0 mM NO$\sb{3-}.$ In leaves, NRA showed a curvilinear response to increasing solution NO$\sb{3-}$ rates, with highest NRA at 5.0 mM solution NO$\sb{3-}.$ At low levels of solution N supply (0.5 and 1.0 mM NO$\sb{3-})$ just about all the N in the leaves of each cultivar was in reduced form, however, as the solution NO$\sb{3-}$ rate increased to 5.0 and 15.0 mM, RN/TN values decreased, and differences among cultivars for in situ NO$\sb{3-}$ assimilation became greater, with RN/TN values higher romaine cultivars than in butterhead cultivars. In the second experiment, tissue NO$\sb{3-}$ concentrations in hydroponically-grown lettuce were reduced significantly with increasing NH$\sb{4+}$:NO$\sb{3-}$ ratios in the nutrient; however, effects of mixed-N nutrition on plant growth response varied considerably, depending on cultivar. With increasing solution NH$\sb{4+}$:NO$\sb{3-}$ ratios up to 50:50 (in a 15.0 mM N solution), shoot NO$\sb{3-}$ concentrations in romaine lettuce at full head development were reduced approximately 58% (52% on a fresh weight basis), while shoot dry weights increased 46% (28% fresh weight). In butterhead cultivars, a solution NH$\sb{4+}$:NO$\sb{3-}$ ratio of 50:50 decreased shoot NO$\sb{3-}$ concentrations 58% (54% fresh weight basis), but shoot growth was also reduced 9% (15% fresh weight basis). In both leaves and roots, NRA decreased with increasing proportion of NH$\sb{4+}$ in the nutrient solution, beginning at a solution NH$\sb{4+}$:NO$\sb{3-}$ ratio of 25:75 in leaves, and 0:100 NH$\sb{4+}$:NO$\sb{3-}$ in roots. In the third experiment, significant interactions between solution flow rate and NO$\sb{3-}$ supply affected shoot NO$\sb{3-}$ concentrations. At 0.5 mM solution NO$\sb{3-},$ leaf NO$\sb{3-}$ concentrations increased with increasing solution flow rate up to 1000 ml min$\sp{-1},$ and then tended to either level off or decrease slightly. In plants supplied with 10.0 mM NO$\sb{3-},$ leaf NO$\sb{3-}$ levels were relatively unaffected by solution flow rate. Lettuce shoot weights increased with increasing solution flow rate up to 1000 ml min$\sp{-1}$ at the Low NO$\sb{3-}$ supply, and up to 25 ml min$\sp{-1}$ in plants grown with 10.0 mM NO$\sb{3-}.$ Both actual and potential NRA in leaves was unaffected by solution flow rate. Although actual NRA in leaves was significantly higher at 10.0 mM solution NO$\sb{3-}$ than at 0.5 mM NO$\sb{3-},$ there was little or no effect of NO$\sb{3-}$ supply rate on potential NRA in leaves. (Abstract shortened by UMI.) Made available in DSpace on 2011-05-07T12:02:18Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9702438.pdf: 4081739 bytes, checksum: ee5b9faac86a732cca4ef5fa89645127 (MD5) Previous issue date: 1996 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:49Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:14:15-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only 9780591087642 AAI9702438 (UMI)AAI9702438 http://hdl.handle.net/2142/19271 eng Copyright 1996 Al-Redhaiman, Khalid Nasser Agriculture, Agronomy Biology, Plant Physiology Nitrate accumulation in hydroponically-grown lettuce cultivars and relationship to nitrogen supply text Agriculture, Agronomy Natural Resources & Environmental Science Horticulture University of Illinois at Urbana-Champaign Dissertation Ph.D.