2026-09-21T03:42:36Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/19324 2023-07-10 col_2142_5131 col_2142_16357 com_2142_5130 com_2142_16356 com_2142_8913 com_2142_8903

Zielinski, Raymond E. Ling, Vincent 2011-05-07T12:03:56Z 2011-05-07T12:03:56Z 10000-01-01 1990 Calmodulin (CaM) is a calcium-binding protein found in eukaryotes, and appears to be essential for growth and development. CaM is thought to play a pivotal role in modulating biochemical pathways in conjunction with transient calcium fluxes into the cytoplasm. In order to better understand calcium-regulated signal transduction pathways in plants at the molecular level, cDNA sequences of CaM and CaM-like proteins were cloned and characterized from higher plants. A barley (Hordeum vulgare L.) leaf cDNA library in lambda phage gt10 was initially screened with a cam-specific synthetic, 14 nucleotide oligonucleotide probe. A full-length cDNA encoding barley CaM was isolated. Using barley CaM cDNA as heterologous DNA probes, three cDNAs were isolated from an Arabidopsis thaliana cDNA library. Two cDNAs encoded CaM isoforms and one cDNA encoded a CaM-like protein (ACaMLP). Northern hybridization analysis reveal differential transcript accumulation of CaM isoforms and calmodulin-like protein in Arabidopsis total RNA. In order to examine effects of CaM action in vivo, tobacco leaf discs (Nicotiana tabacum, cv. Xanthi) were transformed with barley CaM expression vectors driven by a 35-cauliflower mosaic virus promoters. Homozygous R1 plants were bred and were found to contain one- to two-fold increase of CaM protein as compared to normal tobacco. Some aberrant phenotypes were found and are discussed. Made available in DSpace on 2011-05-07T12:03:56Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114320.pdf: 6933022 bytes, checksum: 86395b9296bf1fbceaeef7aa3d50b38b (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:11Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:14:31-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9114320 (UMI)AAI9114320 http://hdl.handle.net/2142/19324 eng Copyright 1990 Ling, Vincent Biology, Molecular Agriculture, Plant Culture Chemistry, Biochemistry Biology, Plant Physiology The cloning of calmodulincDNAs from barley and Arabidopsis and expression of barley calmodulin in transgenic tobacco text Ph.D. Plant Biology Plant Biology University of Illinois at Urbana-Champaign Dissertation