2026-09-23T04:50:39Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/19417 2023-07-10 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Salyers, Abigail A. Nikolich, Mikeljon Peter 2011-05-07T12:06:54Z 2011-05-07T12:06:54Z 10000-01-01 1994 Tetracycline resistance (Tc$\sp{\rm r})$ in the human colonic Bacteroides and in the related genus Prevotella appears to be conferred by a single resistance determinant. This determinant represents a new class of ribosome protection Tc$\sp{\rm r}$ genes which was designated TetQ, because it has a deduced amino acid sequence that is 40% identical to TetM or TetO (which are $>$75% identical). A deep evolutionary relationship was found to exist between the ribosome protection Tc$\sp{\rm r}$ genes and the elongation factors, particularly the EF-G/EF-2 family. The early divergence between these lineages indicates that the ribosome protection Tc$\sp{\rm r}$ genes are of a more ancient origin than was previously believed. Like tetM and tetO, which have been disseminated in diverse bacterial groups, tetQ appears to be spreading by horizontal transmission within the Bacteroides group. Identical tetQ sequences were found in a number of isolates with differing taxonomy and geographic origin, indicating that extensive natural gene transfer has occurred. Among the exchange events indicated by the evidence was the very recent transfer of an allele of tetQ usually found in Prevotella to a Bacteroides fragilis strain. A new tetQ-containing chromosomal element from Bacteroides, Tc$\sp{\rm r}$Em$\sp{\rm r}$ 7853, was capable of this type of broad host range transfer. Tc$\sp{\rm r}$Em$\sp{\rm r}$ 7853 appears to have structural relatives in isolates of Prevotella ruminicola, indicating that a larger family of elements may be maintained in Bacteroides and Prevotella species, and may be capable of transferring antibiotic resistance between the genera in nature. Made available in DSpace on 2011-05-07T12:06:54Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503288.pdf: 4404365 bytes, checksum: 50d182d4975ffd9a06d2d5bf7726d609 (MD5) Previous issue date: 1994 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:36:49Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:14:58-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9503288 (UMI)AAI9503288 http://hdl.handle.net/2142/19417 eng Copyright 1994 Nikolich, Mikeljon Peter Biology, Molecular Biology, Microbiology Tetracycline resistance and its dissemination in the Bacteroides group text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.