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oai:www.ideals.illinois.edu:2142/19534 2023-07-10 col_2142_5131 col_2142_14826 com_2142_5130 com_2142_8913 com_2142_8903

Daniel, William L. Cho, Young-Lan 2011-05-07T12:10:29Z 2011-05-07T12:10:29Z 10000-01-01 1990 Genomic DNA and total RNA was isolated from blastemas of control and retinoic acid (RA) treated animals during the following limb regeneration stages: initial dedifferentiation (ID), medium bud (MB), notch (N), and 3-finger bud (3-FB). Xenopus nuclear rDNA and axolotl 16S mitochondrial rDNA fragments were used to detect rDNA and rRNA during regeneration. Nuclear rDNA was amplified during the MB stage of regeneration in the absence of RA. The amplified rDNA did not appear to be contributing to the 18S and 28S rRNA pools of MB blastemal cells, at least not in direct proportion to the number of copies of amplified rDHA sequences. RA reduced the level of the amplified sequence in MB blastemas and extended its presence through the N stage of regeneration. The 5.4 kb rDNA signal tended to be stronger in blastemas from RA-treated animals. The levels of both 18S and 28S rRNA tended to be higher in ID and MB blastemas from RA- treated animals. A cloned sequence from an axolotl blastemal cDNA library was almost identical to a region of the bovine 16S mitochondrial rDHA sequence and was inferred to be derived from the axolotl mitochondrial 165 rRNA gene. Both axolotl mitochondrial rDNA and 16S rRNA exhibited relatively little variation among regeneration stages between control and RA-treated samples from corresponding stages. RA, within the limits of these experiments, has a more pronounced effect upon nuclear rRNA gene expression than upon the corresponding mitochondrial rRNA gene expression. Made available in DSpace on 2011-05-07T12:10:29Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114203.pdf: 3361836 bytes, checksum: 514c56f5f5444b6abeaf6ec5f7fd5b97 (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:37:41Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:15:32-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9114203 (UMI)AAI9114203 http://hdl.handle.net/2142/19534 eng Copyright 1990 Cho, Young-Lan Biology, Molecular Effects of retinoic acid on ribosomal RNA gene expression during limb regeneration text Biology Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.