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Voss, Edward W., Jr. Gulliver, Gene Allen 2011-05-07T12:19:55Z 2011-05-07T12:19:55Z 10000-01-01 1995 The diverse active site structures of Map 4-4-20 (pocket; anti-fluorescein) and 04-01(cleft; anti-ssDNA) have been studied extensively (Herron et al., 1989; Denzin et al., 1991; Herron et al., 1991; Denzin et al., 1993; Rumbley et al., 1993), and have led to the hypothesis that the unique biochemical properties exhibited by the active sites of these two antibodies are a function of properties intrinsic to their respective heavy chains, as the light chains are nearly identical, while the heavy chains differ significantly (Bedzyk et al., 1990; Smith et al., 1989; Smith and Voss, 1990). In order to investigate this hypothesis, hybrid single chain antibodies were generated by transplanting heavy chain framework and complementarity determining regions (HFRs and HCDRs) from anti-fluorescein SCA 4-4-20 into the H-chain of anti-ssDNA 04-01 as a means to identify those HFRs and HCDRs required to convey the biochemical properties (structure, function, and stability) of diverse antibody active sites, i.e., a cleft versus a pocket. Such analyses yielded significant information concerning the dependence of antibody active site properties on heavy chain primary structure, which could be applied to other systems to aid in the understanding of the relationships between primary structure and the various biochemical properties exhibited by a protein. Made available in DSpace on 2011-05-07T12:19:55Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9543599.pdf: 3858216 bytes, checksum: bde5bfba3425d4c8aa5c0c1ba7f8e9b2 (MD5) Previous issue date: 1995 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:39:42Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:16:47-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9543599 (UMI)AAI9543599 http://hdl.handle.net/2142/19828 eng Copyright 1995 Gulliver, Gene Allen Biology, Microbiology Health Sciences, Immunology Effects of HCDR transplantation on the biochemical properties of diverse antibody active sites text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.