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Mackie, Roderick I. Odenyo, Agnes Awino 2011-05-07T12:23:49Z 2011-05-07T12:23:49Z 10000-01-01 1992 A total of 6 specific oligonucleotide probes were designed for the Ruminococci, 3 targeted to sites on 16S rRNA of Ruminococcus albus and 3 targeted to sites on 16S rRNA of Ruminococcus flavefaciens. A subspecies probe for Fibrobacter succinogenes S85 (Stahl et al., 1988) and a eubacterial kingdom probe (Stahl and Amann, 1991) also were used. Bacteria were grown as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\sp\circ$C for 10 hours and sampled hourly. The cellulose and alkaline hydrogen peroxide-treated wheat straw-grown cultures were incubated at 39$\sp\circ$C for 70 hours and sampled every 8 hours. Ribosomal RNA was extracted from all the samples, blotted on nylon membranes and hybridized with labelled $\sp{32}$P probes. The membranes the were exposed to films. Results showed that R. albus 8 inhibited the growth of R. flavefaciens FD-1 when grown as a diculture on cellubiose or cellulose. However, R. albus 8 was outnumbered by F. succinogenes S85 on cellobiose but not on cellulose. R. flavefaciens FD-1 outnumbered F. succinogenes S85 on both cellobiose and cellulose. In the triculture, R. flavefaciens FD-1 was inhibited, R. albus 8 appeared to dominate during early incubation (12-48 hours) on cellulose, while F. succinogenes S85 became predominant during the late incubation phase (60-70 hours). On alkaline hydrogen peroxide-treated wheat straw, F. succinogenes S85 grew better than either R. albus 8 or R. flavefaciens FD-1. However, R. flavefaciens FD-1 was present in low numbers throughout the incubation period unlike on cellobiose and cellulose. The results on cellobiose and cellulose suggest that R. albus 8 produced a substance that inhibited the growth of R. flavefaciens FD-1. Made available in DSpace on 2011-05-07T12:23:49Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9305642.pdf: 6144968 bytes, checksum: 4fce9e7b1b5dfae2b101be12124131d6 (MD5) Previous issue date: 1992 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:40:30Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:17:23-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9305642 (UMI)AAI9305642 http://hdl.handle.net/2142/19946 eng Copyright 1992 Odenyo, Agnes Awino Biology, Ecology Biology, Microbiology Agriculture, Plant Pathology Molecular ecology of plant cell wall hydrolysis by mixed cultures of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1 and Fibrobacter succinogenes S85 text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.