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Ordal, George W. Kirsch, Michael Lee 2011-05-07T12:29:23Z 2011-05-07T12:29:23Z 10000-01-01 1994 The two enzymes involved in the major adaptation pathway of Bacillus subtilis chemotaxis were cloned and sequenced. They were found to be homologous to the corresponding enzymes of the Escherichia coli system. While the B. subtilis proteins could substitute for the E. coli proteins, they play opposite roles in the two organisms. CheB, the methylesterase, functions to promote adaptation to attractant stimuli in B. subtilis whereas it serves to promote adaptation to repellent stimuli in E. coli. CheR, the methyltransferase, functions to promote adaptation to repellent stimuli in B. subtilis whereas it serves to promote adaptation to attractant stimuli in E. coli. There exists another mechanism in B. subtilis which promotes adaptation to attractant stimuli but is independent of the methylation system. A methylation-independent adaptation system has been described for the E. coli system but it plays a much less significant role in that organism. A cheR null mutant gives rise to two distinct subpopulations of cells which have different chemotactic phenotypes. A single bacterium always develops into the two different subpopulations. The behavior of one of the subpopulations is very similar to that of the double cheB/cheR mutant. Furthermore CheB activity is diminished when CheR is missing, as in the cheR mutant, or when there is an excess of CheR, as in the wild-type strain which overexpresses cheR. Made available in DSpace on 2011-05-07T12:29:23Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503239.pdf: 4170213 bytes, checksum: cf0b57292c9806721c416608c5c20ee0 (MD5) Previous issue date: 1994 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:41:41Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:18:04-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9503239 (UMI)AAI9503239 http://hdl.handle.net/2142/20118 eng Copyright 1994 Kirsch, Michael Lee Biology, Microbiology Chemistry, Biochemistry Genetic, biochemical and behavioral studies of the chemotactic response in Bacillus subtilis text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.