2026-09-21T23:07:47Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/20151 2023-07-10 col_2142_5131 col_2142_14835 com_2142_5130 com_2142_14834 com_2142_14833

Made available in DSpace on 2011-05-07T12:30:25Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9136679.pdf: 3507094 bytes, checksum: d9f1d897d82836c6484f182b3a2c4edb (MD5) Previous issue date: 1991 Whiteley, Herbert E. Morton, Laura Dill 2011-05-07T12:30:25Z 2011-05-07T12:30:25Z 10000-01-01 1991 The pathogenesis of Acanthamoeba keratitis, an increasingly reported and often unmanageable protozoal infection occurring primarily in wearers of soft contact lenses, is unknown. Since adherence of a pathogen to the corneal epithelium is an important initial event in the development of microbial keratitis, a description of factors that influence the adherence of Acanthamoeba to corneal epithelium may be useful in understanding the pathogenesis of amebic keratitis. An in vitro coincubation assay was used to measure adhesion of radiolabeled Acanthamoeba trophozoites to monolayers of rabbit corneal epithelium, monolayers of rabbit corneal stromal fibroblasts, and wounded ex vivo rat corneas. Adhesion of amebae to corneal epithelium occurred rapidly, was higher at 25$\sp\circ$C than at 37$\sp\circ$C or 4$\sp\circ$C, did not totally account for the reported in vivo pathogenicity of the strain of Acanthamoeba, and was inhibited by mannose or methyl-a-D-mannopyranoside. More than twice as many amebic trophozoites adhered to corneal stromal fibroblasts as to corneal epithelial cells. Subtle partial epithelial defects did not augment adherence of Acanthamoeba trophozoites to ex vivo rat corneas. These findings suggest that at temperatures of 25$\sp\circ$C or higher Acanthamoeba organisms can quickly adhere to epithelium or contact lens deposits containing epithelial debris, possibly by recognition of mannose moieties. Stromal exposure appears to augment adherence, suggesting a role for penetrating corneal wounds in the pathogenesis of amebic keratitis. Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:41:54Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:18:11-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9136679 (UMI)AAI9136679 http://hdl.handle.net/2142/20151 eng Copyright 1991 Morton, Laura Dill Health Sciences, Ophthalmology Biology, Microbiology Health Sciences, Pathology Adhesion mechanisms of Acanthamoeba text Veterinary Medicine Veterinary Biosciences University of Illinois at Urbana-Champaign Dissertation Ph.D.