2026-09-21T11:53:05Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/20426 2023-07-10 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Salyers, Abigail A. Suwanto, Antonius 2011-05-07T12:38:51Z 2011-05-07T12:38:51Z 10000-01-01 1992 Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb. The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites. Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium. Made available in DSpace on 2011-05-07T12:38:51Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215894.pdf: 8412906 bytes, checksum: 21a75aac7ce82a4a0881dfe9c3564318 (MD5) Previous issue date: 1992 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:43:49Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:19:13-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9215894 (UMI)AAI9215894 http://hdl.handle.net/2142/20426 eng Copyright 1992 Suwanto, Antonius Biology, Molecular Biology, Genetics Biology, Microbiology Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.