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ETDs are only available to UIUC Users without author permission U of I Only AAI9522186 (UMI)AAI9522186 http://hdl.handle.net/2142/20759 eng Gillette, Martha U. Weber, Edward Todd 2011-05-07T12:48:27Z 2011-05-07T12:48:27Z 10000-01-01 1995 The hypothalamic suprachiasmatic nuclei (SCN) serve as the primary mammalian circadian pacemaker. Measurements from SCN in brain slice preparations from rats reveal circadian rhythms of intracellular levels of cGMP, and in $\rm\lbrack\gamma$-$\rm\sp{32}PO\sb4\rbrack$-orthophosphate incorporation into constant levels of a 75 kD protein in in vitro reactions stimulated by cGMP. This protein was identified as cGMP-dependent protein kinase (PKG) by immunoprecipitation and immunoblots using polyclonal antisera raised against this phosphoprotein extracted and purified from bovine brain. In vitro assays revealed a circadian rhythm of endogenous PKG activity in SCN from samples procured at different points in the circadian cycle which coincided with the rhythm of endogenous cGMP levels and PKG autophosphorylation. In vivo studies were performed in which the nitric oxide synthase inhibitor, L-NAME, was injected into the third ventrical at the level of the SCN, 10 minutes prior to photic stimulation, and was shown to inhibit light-induced phase shifts of locomotor rhythms. The effect was reversible and dose-related, and could be outcompeted by addition of excess arginine. D-NAME did not block photic phase-shifts. Neither L-NAME nor arginine affected phase of rhythms in the absence of light. Arginine did not potentiate light-induced phase shifts. L-NAME did not block light induced expression of the intermediate-early gene product, Fos. Finally, the putative regulation of cAMP-response element binding protein (CREB) by PKG was evaluated using several approaches. CREBtide, a 14-amino acid sequence of CREB surrounding the activation-specific phosphoacceptor ser-133, was phosphorylated by PKG at a rate comparable to that of the PKG-specific heptapeptide substrate sequence of histone used to characterize PKG kinetics. $\rm\lbrack\sp{32}PO\sb4\rbrack$-orthophosphate incorporation into a 43 kD protein corresponding to CREB on immunoblots was demonstrated in SCN following subjective nighttime stimulation with 8-Br-cGMP. Finally, specific phospho-CREB immunostaining in the SCN was significantly blocked by preincubation of the antiserum with CREBtide phosphorylated by PKG. Made available in DSpace on 2011-05-07T12:48:27Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9522186.pdf: 4032597 bytes, checksum: eeeb9d1f946fab5d44d9914246bdbb7c (MD5) Previous issue date: 1995 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:46:04Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:20:34-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission Copyright 1995 Weber, Edward Todd Biology, Animal Physiology The roles of nitric oxide,cGMP, and cGMP-dependent protein kinase in generation and modulation of circadian rhythms by the suprachiasmatic nucleus text Molecular and Integrative Physiology Physiology University of Illinois at Urbana-Champaign Dissertation Ph.D.