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oai:www.ideals.illinois.edu:2142/21113 2023-07-10 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Gardner, Jeffrey F. Lee, Jeong Kug 2011-05-07T12:58:46Z 2011-05-07T12:58:46Z 10000-01-01 1992 The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator. The puc operon encodes 2.3- and 0.5-kb transcripts. The 2.3-kb transcript extends from the same 5$\sp\prime$ end (117 nucleotides from the start of pucB) as that of the 0.5-kb transcript to approximately 1.8 kb downstream of the pucBA genes, and encodes gene product(s) involved in post-translational expression of the pucBA gene products involved in the formation of B800-850 complex. A 629-base pair DNA region upstream of the 5$\sp\prime$-ends of the two puc-specific transcripts encompasses two functionally separable cis-acting domains: the upstream regulatory region (URS, $-$629 to $-$150) responsible for oxygen and light control of puc operon transcription, and the more proximal downstream regulatory region (DRS, $-$150 to $-$1) containing putative IHF/FNR-binding sites ($-$129 $-$105), putative promoter(s) ($-$92 and $-$57), and operator(s) ($-$52 $-$35 and $-$27 $-$10) involved in oxygen control of puc operon transcription. In addition, data reveal a direct interaction between the URS ($-$629 $-$150) and the DRS ($-$150 $-$1). A trans-acting factor involved in O$\sb2$ control of puc operon transcription acted upon the puc URS, while a second trans-acting factor(s) acted upon both the puc URS and DRS. The former trans-acting factor was genetically mapped to a 2.2-kb DNA fragment located within the carotenoid gene cluster, while the genetic map of the second trans-acting factor was localized to a 7.0-kb DNA fragment containing the puhA gene and its flanking DNA (6.3 kb). Made available in DSpace on 2011-05-07T12:58:46Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9215846.pdf: 8265007 bytes, checksum: b6ae4601ec7d5e2cefeb1614596dacef (MD5) Previous issue date: 1992 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:34Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:22:00-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9215846 (UMI)AAI9215846 http://hdl.handle.net/2142/21113 eng Copyright 1992 Lee, Jeong Kug Biology, Molecular Biology, Microbiology Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1 text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.