2026-09-20T03:49:24Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/21141 2023-07-10 col_2142_5131 col_2142_14826 com_2142_5130 com_2142_8913 com_2142_8903

Korban, Schuyler S. Alrefai, Rudaina Hussein 2011-05-07T12:59:35Z 2011-05-07T12:59:35Z 10000-01-01 1993 Apple mosaic virus (ApMV) (Idaho strain) was purified from infected cucumber cotyledons. Virions 3 and 4 (top components in sucrose gradient) were isolated and RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\sp\prime$-end of RNA4 was mapped using the primer extension technique. ApMV RNA4 was found to be 983 nucleotides in length containing open reading frame (ORF) of a 662 nucleotides, which was flanked by 5$\sp\prime$ and 3$\sp\prime$-nontranslated sequence of 55 and 265 nucleotides, respectively. The ORF encodes for a coat protein of a 25,085 Mr. The sequence contains several inverted repeats that could form hair-pin structures. In addition, two palindromic sequence were found in the ORF with a third palindrome in the 3$\sp\prime$-nontranslated region. These structures may be involved in the recognition of RNA by viral replicase or in genome activation by the coat protein. The amino-terminus of ApMV coat protein is basic and rich in Cys, whereas the carboxy-terminus has an even distribution of basic and acidic amino acids, similar to other ilarviruses. "To transform plants with ApMV coat protein gene an Agrobacterium-mediated transformation system was developed for apple ""Royal Gala"" plants. Kanamycin completely inhibited regeneration of untransformed leaf sections on a 1/2 Murashige and Skoog (MS) regeneration medium. Apple leaf sections were transformed by Agrobacterium strains C58Z707:pZA-7 and EHA101:pZA-7 and calli were produced on a 1/2MS selection medium." Made available in DSpace on 2011-05-07T12:59:35Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9314835.pdf: 3055004 bytes, checksum: 6709f0de44347cc4a866c81095f90774 (MD5) Previous issue date: 1993 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:48:46Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:22:07-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9314835 (UMI)AAI9314835 http://hdl.handle.net/2142/21141 eng Copyright 1993 Alrefai, Rudaina Hussein Agriculture, Agronomy Biology, Molecular Agriculture, Plant Pathology Characterization and nucleotide sequence of apple mosaic virus coat protein gene text Biology Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.