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DeVries, Arthur L. Hsiao, Ku-Chuan 2011-05-07T13:04:21Z 2011-05-07T13:04:21Z 10000-01-01 1990 The antarctic fish, Notothenia neglecta, synthesizes a series of 8 different-sized antifreeze glycopeptides (AFGP 1 to 8) which lower the freezing point of its body fluids below that of sea water. I report here the sequence of one of its AFGP genes. The structural gene contains 46 tandemly repeated segments, each coding for one mature peptide. Most of the repeats (44) are composed of 51 nucleotides and encode peptides of the AFGP 8 class, but the remaining two are longer (60 nucleotides each) and code for peptides of the AFGP 7 types. Separating each repeat are three triplets that code for amino acid residues that are absent in the mature peptides. The expressed nucleotide sequence between the initiation codon and the first AFGP-coding segment is GT-rich and codes for a presumptive hydrophobic signal peptide of unusual sequence (including a long stretch of alternating cysteine and valine residues). This presumptive signal peptide can be partially cleaved in an in vitro processing system. Putative CCAAT and TATA boxes begin 102 and 92 nucleotides, respectively, upstream from the initiation codon, and the polyadenylation signal, AATAAA, is located approximately 240 nucleotides downstream from the termination codon. Thus this AFGP gene appears to encode a secreted, high-copy-number polyprotein that is processed post-translationally to produce active AFGP. Made available in DSpace on 2011-05-07T13:04:21Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114269.pdf: 2744890 bytes, checksum: 2673e322fd4dfb0090fd8719932df5a1 (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:49:46Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:22:40-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9114269 (UMI)AAI9114269 http://hdl.handle.net/2142/21291 eng Copyright 1990 Hsiao, Ku-Chuan Biology, Molecular Biology, Animal Physiology Agriculture, Fisheries and Aquaculture "Characterization of an antifreeze glycopeptide gene from the Antarctic cod, Notothenia neglecta, and a functional study of the ""pre-"" sequence of this antifreeze glycopeptide" text Ph.D. Molecular and Integrative Physiology Physiology University of Illinois at Urbana-Champaign Dissertation