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Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:51:00Z Item is restricted indefinitely. Rittmann, Bruce E. Saez Ramila, Pablo Baldomero 2011-05-07T13:09:34Z 2011-05-07T13:09:34Z 10000-01-01 1990 The kinetic effects of the presence of one substrate on the degradation rate of the other substrate in a single-species, bisubstrate system were quantified in detail. The system consisted of a pure culture (Pseudomonas putida P$\sb{\rm p}$G4 (ATCC 17453)) transforming phenol and 4-chlorophenol simultaneously under fully aerobic conditions. Phenol behaved as a self-inhibitory primary substrate, whose biodegradation rate when present as a single substrate could be modeled well using Haldane Kinetics. 4-chlorophenol behaved as a co-metabolite, because its transformation, observed at a significant rate, did not yield any increases in biomass. When phenol and 4-chlorophenol were present together, the 4-chlorophenol- transformation rate was proportional to the phenol-oxidation rate, because the electrons required for the transformation of 4-chlorophenol were provided by the oxidation of phenol. In the absence of phenol, the 4-chlorophenol-transformation rate was proportional to the biomass-decay rate, because the required electrons were then provided by the biomass oxidation. These proportionalities in the rates clearly indicated that 4-chlorophenol transformation was possible only in the presence of a source of electrons, such as phenol or biomass. 4-chlorophenol inhibited phenol biodegradation. Two types of inhibitory effects were observed. For experiments with low initial 4-chlorophenol/phenol (I/S) ratios, the inhibition mainly decreased the Haldane qm$\sb{\rm s}$ parameter. On the other hand, the inhibition for high initial I/S ratios mainly was expressed by an increase to the Haldane K$\sb{\rm s}$ parameter for phenol. Similarly to the 4-chlorophenol-alone systems, the controlling variable for the type of inhibition observed in the bisubstrate system was the 4-chlorophenol/biomass ratio at the time when phenol oxidation stopped. In summary, phenol was absolutely required for the transformation of 4-chlorophenol, because this transformation required electrons for regenerating the NADPH consumed. The requirement for phenol can be direct, manifested in the actual presence of phenol, or indirect, manifested in the cell mass grown previously when phenol was present (the cells acted as an electron reservoir in this case). 4-chlorophenol, in turn, was inhibitory to the degradation of phenol and to its own transformation. Made available in DSpace on 2011-05-07T13:09:34Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114397.pdf: 8194223 bytes, checksum: 4e95b2a3a4dc5dd8c70d2f422d171268 (MD5) Previous issue date: 1990 Restriction data tranferred 2014-07-01T11:23:22-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9114397 (UMI)AAI9114397 http://hdl.handle.net/2142/21470 eng Copyright 1990 Saez Ramila, Pablo Baldomero Biology, Microbiology Engineering, Sanitary and Municipal Environmental Sciences Quantification of interactions between inhibitory primary and secondary substrates text Civil and Environmental Engineering Environmental Engineering University of Illinois at Urbana-Champaign Dissertation Ph.D.