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oai:www.ideals.illinois.edu:2142/21758 2023-07-10 col_2142_5131 col_2142_14798 com_2142_5130 com_2142_686 com_2142_397

Liebl, Rex A. Koppatschek, Fritz K. 2011-05-07T13:18:15Z 2011-05-07T13:18:15Z 10000-01-01 1989 The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil. Antibodies, specific to clomazone, were produced in New Zealand white rabbits to a BSA-clomazone conjugate. Attempts at producing antibodies specific to imazaquin, via BSA-imazaquin and BSA-hexanoic acid-imazaquin conjugates, were not successful. Antibodies specific to metribuzin, via a porcine gamma globulin-metribuzin conjugate, were not successfully produced. The antibodies to the BSA-clomazone conjugate were sensitive to clomazone having an I$\sb{50}$ of 12 ng/mL. The antibodies were also specific to clomazone and demonstrated no cross reactivity to the soybean herbicides metribuzin, metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide) or trifluralin (2,6-dinitro-N,N-dipropyl-4-(trifluromethyl)benzenamine). Soil extracts, from several different soil types, did not interfere with detection of clomazone in this ELISA. Soil extraction procedures could also be simplified without reducing the sensitivity of the assay. The ELISA system compared favorably with conventional clomazone detection systems of wheat bioassay and gas chromatography. The low level of detection for both gas chromatography and ELISA was from a 10 ppb acetonitrile extract of clomazone treated soil. Clomazone-treated soil was extracted with phosphate buffer and clomazone was quantified with ELISA. Detection of clomazone in the phosphate buffer extracts was influenced by soil type, with greater detection in low organic matter soil. This technique offers a novel method for quantifying a portion of the biologically available herbicide. The ELISA system proved to be a fast, accurate and sensitive method of clomazone detection. Made available in DSpace on 2011-05-07T13:18:15Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9010922.pdf: 2368570 bytes, checksum: 6c7b0373bc0bd66dfc8022fc3bdcff08 (MD5) Previous issue date: 1989 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:53:00Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:24:28-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9010922 (UMI)AAI9010922 http://hdl.handle.net/2142/21758 eng Copyright 1989 Koppatschek, Fritz K. Agriculture, Agronomy Chemistry, Agricultural Enzyme-linked immunosorbent assay for herbicide detection text Crop Sciences Crop Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.