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(UMI)AAI9712410 Lazarowitz, Sondra G. Qin, Shenwei 2011-05-07T13:32:50Z 2011-05-07T13:32:50Z 10000-01-01 1996 Both genomic components of bipartite geminiviruses encode functions essential for viral infection: the A component replicates the viral genome and the B component systemically spreads the virus throughout the host. Thus, correct recognition between the two components is required to ensure a constant co-presence of the two viral genomic components during viral infection. In this study, using two closely related yet sufficiently different strains of squash leaf curl virus (SqLCV) as a model system, I will show that: (1) viral replication is an important step in component recognition of SqLCV infection; (2) the sequence between the directly repeated motif and the stem-loop structure of SqLCV replication origin is involved in component recognition and contains specific sequence element for efficient viral replication; (3) replication protein AL1 interacts with the sequence between the directly repeated motif and the stem-loop structure to balance replication of the two viral components; (4) balanced replication of the two viral components constitutes the molecular basis of component recognition of SqLCV. Separately, I will also study the mechanism by which the coat protein AR1 participates in the viral movement pathway using mutational analysis. I will show that (1) viral encapsidation is not linked to viral ssDNA accumulation; (2) the ability of AR1 to bind ssDNA, possibly cooperatively, is essential for its participation in viral movement. This work will suggest that AR1 switches the viral rolling circle replication to the production of ssDNA to provide a sufficient amount of viral genomes to ensure an efficient viral infection. Made available in DSpace on 2011-05-07T13:32:50Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9712410.pdf: 5197337 bytes, checksum: b74aba28be789f591aa6aca02f15ed5b (MD5) Previous issue date: 1996 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:56:08Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:26:13-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only 9780591200393 AAI9712410 http://hdl.handle.net/2142/22218 eng Copyright 1996 Qin, Shenwei Biology, Molecular Biology, Microbiology The mechanism of component recognition of bipartite geminiviruses: Genetic studies of the replication of squash leaf curl virus, and, Genetic and functional studies of squash leaf curl virus coat protein AR1 text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.