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oai:www.ideals.illinois.edu:2142/22681 2023-07-10 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Somatolactogenic hormones and interferon-gamma as regulators of neutrophil activation Kelley, Keith W. Fu, Yung-Kang 2011-05-07T13:47:54Z 2011-05-07T13:47:54Z 10000-01-01 1991 Experiments described in this thesis investigated the role of two somatolactogenic hormones, growth hormone (GH) and prolactin (PRL), as well as the classic phagocyte activator, interferon-gamma (IFN-$\gamma$), in activating polymorphonulear neutrophils (PMN) from pigs, cows, rats and humans to kill Escherichia coli and secrete superoxide anion (O$\sb2\sp-$) after stimulation with phorbol myristate acetate (PMA). GH, as well as its growth promoting peptide, insulin-like growth factor-I (IGF-I), were both comparable to IFN-$\gamma$ in augmenting the secretion of O$\sb2\sp-$ by PMN. These results indicated that the IGF-I receptor was not essential for GH-mediated priming. However, PRL was able to prime human PMN as well as human GH, so the hypothesis was explored that GH primes human PMN by binding to PRL receptors. All of these data suggested that the PRL receptor rather than the GH receptor was responsible for the GH-mediated priming of human PMN for an enhanced respiratory burst. Since GH secretion declines with advancing age, the effector activity of PMN from young and aged rats in response to both GH and IFN-$\gamma$ was compared. PMN from young rats demonstrated a higher capacity for oxygen-dependent killing of E. coli than those from the aged rats. These results defined an intrinsic defect in PMN from aged rats by showing that their PMN were less sensitive to phagocyte activators and that IFN-$\gamma$ and GH synergized to restore this defective response of PMN and potentiated bactericidal activity. Made available in DSpace on 2011-05-07T13:47:54Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210806.pdf: 5927922 bytes, checksum: f280b703d8d012668c44041c78eba2a4 (MD5) Previous issue date: 1991 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:59:16Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:27:56-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9210806 (UMI)AAI9210806 http://hdl.handle.net/2142/22681 eng Copyright 1991 Fu, Yung-Kang Health Sciences, Immunology text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.