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Wolfe, R.S. Olson, Karl Daniel 2011-05-07T13:57:19Z 2011-05-07T13:57:19Z 10000-01-01 1991 The final step of methane formation in Methanobacterium thermoautotrophicum is a reductive demethylation of 2-(methylthio)ethanesulfonic acid (CH$\sb3$-S-CoM) with reducing equivalents from 7-(mercaptoheptanoyl)-L-threonine O$\sp3$-phosphate (HS-HTP). The enzyme that catalyzes this step is the CH$\sb3$-S-CoM reductase. Bound to the enzyme (non-covalently) is the prosthetic group, native coenzyme F430, a unique nickel-containing tetrapyrrole. Coenzyme F430 and a structural analogue were purified to homogeneity and studied by several types of two-dimensional (2D) nuclear magnetic resonance (NMR) techniques. Modelling studies using 2D nuclear Overhauser effect spectroscopy (NOESY) data, and distance geometry (DG) and back-calculations were used to determine 3-D structural aspects of these molecules. It was also found that inactive CH$\sb3$-S-CoM reductase can be partially activated in the presence of light. This is a major simplification of the terminal step. Interestingly, methanogens were found to be sensitive to visible light (in blue end of the spectrum). Experiments were completed to determine the experimental mid-point potentials electrochemically. These experiments are described. Made available in DSpace on 2011-05-07T13:57:19Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9210943.pdf: 5460559 bytes, checksum: c8413096c9e3ea34872ccc2c567078b4 (MD5) Previous issue date: 1991 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:01:13Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:29:02-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9210943 (UMI)AAI9210943 http://hdl.handle.net/2142/22961 eng Copyright 1991 Olson, Karl Daniel Biology, Microbiology Chemistry, Biochemistry Biochemical and chemical studies of the components involved in the final step of methane formation in Methanobacterium text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.