2026-09-21T19:09:54Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/23303 2023-07-10 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

(UMI)AAI9114429 http://hdl.handle.net/2142/23303 eng Copyright 1990 Sun, Ke-Qin Chemistry, Biochemistry A study of DNA-DNA interactions during bacteriophage lambda integrative recombination text Gumport, Richard I. Sun, Ke-Qin 2011-05-07T14:09:22Z 2011-05-07T14:09:22Z 10000-01-01 1990 An in vitro integrative recombination system was used to study DNA-DNA interactions during site-specific recombination in bacteriophage lambda. Oligodeoxynucleotides 28 bases long containing either the top strand or the bottom strand sequences of the bacterial attachment site (attB) with or without base substitutions in the core region were chemically synthesized. Annealed oligodeoxynucleotide attB sites containing sequences with perfect base pairs or one mismatched base apposition were shown to be proficient in integrative recombination. Supercoiled circular DNA molecules 713 base pairs long containing bacteriophage lambda attachment (attP) sites were constructed by cloning, annealing of the single strands, restriction digestion, and intramolecular ligation. These attP sites, with or without a mismatched base pair in the core region, are also proficient in integrative recombination. Recombination between perfectly base paired attB and supercoiled attP containing mismatched base pairs in the core region was less efficient than that between the wild-type att sites indicating that mismatches in the core region of attP are disrupting normal interactions. Recombination with attP and attB substrates, both containing mismatched base pairs in the overlap region, demonstrated that sequence homology at positions -2 or 0 is not required for the first strand exchange during integrative recombination. The sequence homology at those positions is, however, required for branch migration of the Holliday-structure intermediate formed by the first strand exchange. The results also indicate that the first strand exchange reaction is reversible during the integrative recombination. Made available in DSpace on 2011-05-07T14:09:22Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9114429.pdf: 6014306 bytes, checksum: 6d5f173dec6711c1454205f03bfe7b03 (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:03:34Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:30:18-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9114429 Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.