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Morton, Daniel George 2011-05-07T14:15:11Z 2011-05-07T14:15:11Z 10000-01-01 1990 Porcine pulmonary intravascular macrophages were isolated from six to eight week old pigs by perfusing the pulmonary vasculature with collagenase solution. The isolated cells were nonspecific esterase positive, phagocytic, and had morphologic features of macrophages. Isolated intravascular macrophages adhered in greater numbers to cultured porcine endothelial cells than to porcine fibroblasts, PK-15 porcine kidney epithelial cells, plastic, or basement membrane material. In contrast, alveolar macrophages adhered in greater numbers to plastic surfaces than to any of the cellular substrates. Adherence of intravascular macrophages to endothelial cells was inhibited by coculturing intravascular macrophages with indomethacin or pretreating intravascular macrophages with indomethacin before coculturing the cells with endothelium. Pretreatment of endothelial cells with indomethacin did not inhibit adherence of intravascular macrophages to endothelium. These results suggest that cyclooxygenase products of arachidonic acid from intravascular macrophages are important in modulating adherence of intravascular macrophages or their precursors to endothelium. A23187 added to cocultures enhanced adherence of intravascular macrophages to plastic, but not to endothelium. Pretreatment of either cell type with A23187 did not alter detectably adherence of intravascular macrophages to endothelial cells. Treatment of intravascular macrophages or endothelial cells with bacterial lipopolysaccharide and dexamethasone did not inhibit or stimulate adhesion of intravascular macrophages to endothelium. Intravascular macrophages incubated with aortic and pulmonary arterial explants for four or eight hours formed intercellular adhesion plaques in vitro. Made available in DSpace on 2011-05-07T14:15:11Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9021731.pdf: 4734175 bytes, checksum: 2284285dd68e02bb05c261c275f30437 (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:04:39Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:30:54-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9021731 (UMI)AAI9021731 http://hdl.handle.net/2142/23465 eng Copyright 1990 Morton, Daniel George Biology, Veterinary Science Adhesion and intercellular junction formation by pulmonary intravascular macrophages and endothelial cells in vitro text Pathobiology Pathobiology University of Illinois at Urbana-Champaign Dissertation Ph.D.