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oai:www.ideals.illinois.edu:2142/23540 2023-07-10 col_2142_5131 col_2142_14826 com_2142_5130 com_2142_8913 com_2142_8903

Made available in DSpace on 2011-05-07T14:18:00Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9503198.pdf: 4832446 bytes, checksum: 561998f59f31bfc6ae6319424788015c (MD5) Previous issue date: 1994 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:05:10Z Item is restricted indefinitely. Widholm, Jack M. Goldstein, Cindy Susan 2011-05-07T14:18:00Z 2011-05-07T14:18:00Z 10000-01-01 1994 Photoautotrophic cells are chlorophyllous plant cells grown with no sugars and minimal or no organic carbon in the culture medium. These cells are photosynthetic and are capable of light-dependent CO$\sb2$ fixation. Our laboratory has initiated and continued to grow several different species of photoautotrophs under strict photoautotrophic conditions including Amaranthus powellii, Datura innoxia, Glycine max, and Gossypium hirsutum cell lines, as well as a Nicotiana tabacum-glutinosa fusion hybrid line. These cells have high levels of chiropodies, ranging from approximately 500 $\mu$g/g fresh weight for the photoautotrophic Nicotiana cells to as high as 4000 $\mu$g/g fresh weight for G. max and G. hirsutum cells. The chlorophyll contents of these cells have reached levels much higher than that described in the literature for other photoautotrophic culture lines. The photoautotrophic cells primarily carry out C$\sb3$ photosynthesis, fixing CO$\sb2$ primarily via RuBP carboxylase through the Calvin Cycle. Culture lines often had high levels of PEP carboxylase activity in the first year following culture initiation, with a decrease in PEP carboxylase activity and an increase in RuBP carboxylase activity over a seven to ten year time period. The ratio of RuBP carboxylase activity to PEP carboxylase activity has likewise increased the longer the cells have been selected for continuous culture in a photoautotrophic environment. The percent activation of RuBP carboxylase has increased from activation levels of below 50% to levels of 70-80% for all of the photoautotrophic culture lines except D. innoxia. The photosynthetic capacity of the cells has increased over a 5 to 7 year time period, as well, with increasing rates of carboxylase activity as chlorophyll content has continued to increase. Restriction data tranferred 2014-07-01T11:31:12-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9503198 (UMI)AAI9503198 http://hdl.handle.net/2142/23540 eng Copyright 1994 Goldstein, Cindy Susan Biology, Botany Agriculture, Plant Culture Biology, Plant Physiology Characterization of six photoautotrophic plant cell lines and assessment of changes after long-term continuous culture text Biology, Botany Agriculture, Plant Culture Biology, Plant Physiology Biology, Botany Agriculture, Plant Culture Biology, Plant Physiology University of Illinois at Urbana-Champaign Dissertation Ph.D.