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oai:www.ideals.illinois.edu:2142/23829 2023-07-10 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Gennis, Robert B. Newton, Gail Ann 2011-05-07T14:28:40Z 2011-05-07T14:28:40Z 10000-01-01 1990 The cytochrome d terminal oxidase is one of two terminal oxidases in the aerobic respiratory chain of E. coli. The topology of the two subunits of the complex were examined by the use of alkaline phosphatase gene fusions, and models proposed. The expression and assembly of the subunits was also examined. It was found that subunit II of the complex can be expressed independently of subunit I and associates with the cytoplasmic membrane. It does not appear to have any of the cytochrome components associated with it under these conditions. The plasmid expressing subunit II complements another plasmid expressing subunit I, restoring oxidase activity. The incorporation of the $b\sb{595}$ and $d$ cytochrome components requires the association of the two subunits. It has also been confirmed that the expression of the cytochrome $d$ complex is not regulated by the presence or absence of heme. A model for the assembly of the complex has been proposed. Made available in DSpace on 2011-05-07T14:28:40Z (GMT). No. of bitstreams: 2 license.txt: 4922 bytes, checksum: 910b249b4beec47e7ab768910c8f966f (MD5) 9026280.pdf: 2406969 bytes, checksum: 03d6ffc4e13a6e76726d130b9ea94d1d (MD5) Previous issue date: 1990 Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T15:07:07Z Item is restricted indefinitely. Restriction data tranferred 2014-07-01T11:32:16-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission ETDs are only available to UIUC Users without author permission U of I Only AAI9026280 (UMI)AAI9026280 http://hdl.handle.net/2142/23829 eng Copyright 1990 Newton, Gail Ann Chemistry, Biochemistry Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by genetic methods text Dissertation Ph.D. Chemistry, Biochemistry Chemistry, Biochemistry University of Illinois at Urbana-Champaign